Protein Info for PS417_02485 in Pseudomonas simiae WCS417

Annotation: tRNA delta(2)-isopentenylpyrophosphate transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR00174: tRNA dimethylallyltransferase" amino acids 7 to 305 (299 residues), 335.1 bits, see alignment E=1.7e-104 PF01715: IPPT" amino acids 41 to 294 (254 residues), 294 bits, see alignment E=9.8e-92

Best Hits

Swiss-Prot: 97% identical to MIAA_PSEFS: tRNA dimethylallyltransferase (miaA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 97% identity to pfs:PFLU0519)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TUI0 at UniProt or InterPro

Protein Sequence (323 amino acids)

>PS417_02485 tRNA delta(2)-isopentenylpyrophosphate transferase (Pseudomonas simiae WCS417)
MSALPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRDMDIGTAKPSKALLAQY
PHQLIDIIDPSESYSAADFRTDALAAMADITARGNIPLLVGGTMLYYKALQEGLADMPPA
DAQVRAELEEEAARLGWQALHDQLASVDPISAARIHPNDPQRLTRALEVWRVSGQTMTEH
RLKQSAQSADAGASGASQLPYTVANLAIAPANRQVLHERIAQRFTIMLEQGFVDEVVALR
SRGDLHPGLPSIRAVGYRQVWDHLDGKLTSAEMQERGIIATRQLAKRQFTWLRSWSDLHW
LDSLDSDNLSRALKYLGTVSILS