Protein Info for GFF4866 in Xanthobacter sp. DMC5

Annotation: Protein HesB, vegetative

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 104 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details PF01521: Fe-S_biosyn" amino acids 1 to 97 (97 residues), 41.8 bits, see alignment E=5.8e-15 TIGR00049: iron-sulfur cluster assembly accessory protein" amino acids 4 to 99 (96 residues), 65.5 bits, see alignment E=2.2e-22

Best Hits

Swiss-Prot: 35% identical to Y1755_BRADU: Uncharacterized protein blr1755 (blr1755) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: None (inferred from 90% identity to xau:Xaut_0113)

Predicted SEED Role

"probable iron binding protein from the HesB_IscA_SufA family in Nif operon" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (104 amino acids)

>GFF4866 Protein HesB, vegetative (Xanthobacter sp. DMC5)
MNFTMTRSAARFMRMMIMADGGPTAGFRMHVTPGGCSGLNADISVLPEPQPGDVVVEKDG
VKLFLPAESRLLLDGVTIDFTDTAAKTGLVFNDPKATTCSSHAH