Protein Info for GFF4864 in Xanthobacter sp. DMC5

Annotation: FeMo cofactor biosynthesis protein NifB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 TIGR01290: nitrogenase cofactor biosynthesis protein NifB" amino acids 58 to 491 (434 residues), 685.7 bits, see alignment E=1.2e-210 PF04055: Radical_SAM" amino acids 84 to 262 (179 residues), 73 bits, see alignment E=3.3e-24 PF02579: Nitro_FeMo-Co" amino acids 400 to 488 (89 residues), 73.7 bits, see alignment E=1.2e-24

Best Hits

Swiss-Prot: 79% identical to NIFB_HERSE: FeMo cofactor biosynthesis protein NifB (nifB) from Herbaspirillum seropedicae

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 95% identity to xau:Xaut_0111)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>GFF4864 FeMo cofactor biosynthesis protein NifB (Xanthobacter sp. DMC5)
MHASTQQENAVGLNAVGMDEKLAEVMQAKAEHQGCGTSGGSGKASCGSSAGQGDLPTEIW
EKVKNHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAA
KKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFELIAKTAPDIKLCLSTNGLALPDHV
DTIAGFNVDHVTITINMVDPEVGAKIYPWVFWKHKRYTGYEAAKLLTDRQMLGLEMLTER
GILCKVNSVMIPGINDKHLVEVNRAVKSRGAFLHNIMPLISSPEHGTVFGLNGQRGPTAQ
ELKALQDECEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDLDSRKAYQA
LVEEERVAKVAAKQEELEALAGEQSDIKLLTAVATKGSGLINEHFGHAKEFQVWELSTAG
AKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKADLIKAGIEPVDTF
AHEFIEKSAITWFKEYLNKVKTGEIVHTARGDAEIRQGALINAA