Protein Info for GFF4852 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Adenylosuccinate synthetase (EC 6.3.4.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00709: Adenylsucc_synt
  • amino acids 15 to 454 (440 residues), 570.3 bits, E=1.2e-175
• TIGR00184: adenylosuccinate synthase
  • amino acids 15 to 460 (446 residues), 531.1 bits, E=1e-163

Best Hits

Swiss-Prot: 83% identical to PURA_DELAS: Adenylosuccinate synthetase (purA) from Delftia acidovorans (strain DSM 14801 / SPH-1)

KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 83% identity to del:DelCs14_1606)

Predicted SEED Role

"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.4

Protein Sequence (462 amino acids)

>GFF4852 Adenylosuccinate synthetase (EC 6.3.4.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKNSSSAVTPGRNVVVVGTQWGDEGKGKLVDWLTETATGVVRFQGGHNAGHTLVINGVKT
ALHLIPSGIMRAGVKCYIGNGVVLSVSKLFEEIAGLEKAGVEVRSRLRVSEGCPLILPFH
AAIDIAREAAKVKAGTEKIGTTGRGIGPAYEDKIARRALRVQDLKYPDRFATKLRELLDL
HNHVLTSYLHSADMAWDDKLASYMKDGAIQFEPVYAEAMQQAELLKPMIADVSRELNAAH
QAGANLLFEGAQGTLLDIDHGTYPFVTSSNCVAGNAAAGSGVGPGLLHYVLGITKAYCTR
VGGGPFPTELEWEKEGTPGWHMATVGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLC
ITKLDVLDGLKELQLCTGYELDGEVIDILPMSAEDIARCKPIYETLPGWSDSSVGATRYE
DLPVNAQRYLTRIAETTGVPIHIVSTSPDRDHTILLHNPYHG