Protein Info for GFF4839 in Sphingobium sp. HT1-2

Annotation: Threonine dehydrogenase and related Zn-dependent dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 PF08240: ADH_N" amino acids 25 to 144 (120 residues), 81.2 bits, see alignment E=6.1e-27 PF00107: ADH_zinc_N" amino acids 186 to 318 (133 residues), 43.8 bits, see alignment E=2.4e-15

Best Hits

Swiss-Prot: 44% identical to FADH_PSEPU: Glutathione-independent formaldehyde dehydrogenase (fdhA) from Pseudomonas putida

KEGG orthology group: None (inferred from 75% identity to ppg:PputGB1_3009)

Predicted SEED Role

"Threonine dehydrogenase and related Zn-dependent dehydrogenases" in subsystem Threonine degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>GFF4839 Threonine dehydrogenase and related Zn-dependent dehydrogenases (Sphingobium sp. HT1-2)
VKAVVYNGPKDVSVSTIDDPKIEKQTDVIIRLTTTNICGSDLHMYEGRTSFEKGRVFGHE
NLGEVIEVGSAVDRIKKGDRVCMPFNVGCGFCENCERGLTGFCLTTNPGTAGAAYGFAEM
GPWQGGQAELMRVPYADFNCLKLPEDAEEKEDDYVMLSDIFPTGWHGVELSGFLPGESIA
IYGAGPVGLMAAHSAEIRGASQIFVVDSHDDRLKLAKAMGAIPIDMRKGDPAQQILDATS
GLGTDRGVEAVGYQCCDRHGKERSNYTMNCLVKSTKATGGIGVVGVFIPEDPNAPDDLQK
EGKMAFDFGNFWFKGQKIGTGQCNVKHYNRRLMKLIEQGRANPAQIISHRLPLDQAPDAY
KHFDERDAGWTKVVLKPGT