Protein Info for PS417_24745 in Pseudomonas simiae WCS417

Annotation: gamma-glutamyl phosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 14 to 282 (269 residues), 52.7 bits, E=1.3e-18
  • amino acids 314 to 377 (64 residues), 25.3 bits, E=2.7e-10
• TIGR00407: glutamate-5-semialdehyde dehydrogenase
  • amino acids 16 to 410 (395 residues), 508.6 bits, E=5.4e-157

Best Hits

Swiss-Prot: 99% identical to PROA_PSEFS: Gamma-glutamyl phosphate reductase (proA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 99% identity to pfs:PFLU5427)

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

• L-citrulline biosynthesis (8/8 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-N^δ-acetylornithine biosynthesis (6/7 steps found)
• L-ornithine biosynthesis II (3/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Sequence Analysis Tools

See A0A1N7UCM2 at UniProt or InterPro

Protein Sequence (421 amino acids)

>PS417_24745 gamma-glutamyl phosphate reductase (Pseudomonas simiae WCS417)
MTESVLDYMTRLGRAARQASRLIARASTAQKNRALLAAADALDASRSELTAANEQDLANG
RANGLEPALLDRLALTPARIDDMIEGLRQVAKLPDPIGEIRDMRYLPSGIQVGKMRVPLG
VIGIIYESRPNVTIDAASLCLKSGNATILRGGSEAINSNRAIAACIQQGLAVAELPAEVV
QVVETTDRAAVGALITMPEFVDVIVPRGGKSLIERVSRDAKVPVIKHLDGVCHVYIDIAA
DIDKAIRIADNAKTHRYAPCNTMETLLVHVGIADRVLPPLAAIYRDKGVELRGCGRTRAL
LGADVIDATELDWYTEYTAPILSIKIVDDLDEAIEHINTYGSKHTDAIVSEHFSDARRFL
TEVDSASVMINASTRFADGFEYGLGAEIGISTDKLHARGPVGLEGLTSEKYVVFGDGHVR
T