Protein Info for PS417_24695 in Pseudomonas simiae WCS417

Annotation: lipoate--protein ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 99% identical to LIPB_PSEFS: Octanoyltransferase (lipB) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03801, lipoyl(octanoyl) transferase [EC: 2.3.1.181] (inferred from 99% identity to pfs:PFLU5417)

MetaCyc: 58% identical to lipoyl(octanoyl) transferase (Escherichia coli K-12 substr. MG1655)
Lipoyl(octanoyl) transferase. [EC: 2.3.1.181]; RXN0-1138 [EC: 2.3.1.181]

Predicted SEED Role

"Octanoate-[acyl-carrier-protein]-protein-N-octanoyltransferase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

lipoate biosynthesis and incorporation I (2/2 steps found)
lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)

KEGG Metabolic Maps

Lipoic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.181

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UCK7 at UniProt or InterPro

Protein Sequence (215 amino acids)

>PS417_24695 lipoate--protein ligase (Pseudomonas simiae WCS417)
MSHVLGFRELGRMDYEPVWHAMQRFTNERGTSAPDEIWLVEHPPVFTQGQAGKAEHLLLP
GDIPVVQVDRGGQVTYHGPGQLVAYLLLDVRKLGFGVRDLVSRMEACLIELLASYGVSAA
AKPDAPGVYVDGAKIASLGLRIRHGCSFHGLALNVDMDLAPFRRINPCGYAGLAMTQLSD
HATPIKFAEVSARLRAQLVKHLDYAEQTTLTGGID