Protein Info for PS417_24690 in Pseudomonas simiae WCS417

Annotation: lipoyl synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16881: LIAS_N
  • amino acids 50 to 90 (41 residues), 32 bits, 1.4e-11
• TIGR00510: lipoyl synthase
  • amino acids 50 to 331 (282 residues), 431.4 bits, E=9.5e-134
• PF04055: Radical_SAM
  • amino acids 105 to 268 (164 residues), 77.5 bits, E=1.4e-25

Best Hits

Swiss-Prot: 99% identical to LIPA_PSEFS: Lipoyl synthase (lipA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 99% identity to pfs:PFLU5416)

MetaCyc: 66% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

• lipoate biosynthesis and incorporation I (2/2 steps found)
• lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
• lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
• lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)
• lipoate biosynthesis and incorporation II (1/3 steps found)

KEGG Metabolic Maps

• Lipoic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Sequence Analysis Tools

See A0A1N7U484 at UniProt or InterPro

Protein Sequence (341 amino acids)

>PS417_24690 lipoyl synthase (Pseudomonas simiae WCS417)
MTTDAVQTMIPTVDVTERPAPAPRAKVEAGVKLRGAEKVARIPVKIIPTTELPKKPDWIR
VRIPVSPEVDRIKALLRKHKLHSVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDV
GHGRPKPLDVNEPESLAIAIADLKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLSPNV
MLETLVPDYRGRMEVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQKFKQ
MMPHIPTKSGLMLGLGETDEEVIEVMKRMREHDIDMLTLGQYLQPSRSHLPVQRFVHPDT
FAWFAEEGYKMGFKNVASGPLVRSSYHADEQAKLVKASLVS