Protein Info for GFF482 in Variovorax sp. SCN45

Annotation: Glycine betaine/L-proline transport substrate-binding protein ProX (TC 3.A.1.12.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF04069: OpuAC" amino acids 45 to 319 (275 residues), 158.3 bits, see alignment E=1.5e-50

Best Hits

Swiss-Prot: 62% identical to OUSX_DICD3: Glycine betaine-binding periplasmic protein OusX (ousX) from Dickeya dadantii (strain 3937)

KEGG orthology group: K02002, glycine betaine/proline transport system substrate-binding protein (inferred from 92% identity to vap:Vapar_2519)

MetaCyc: 60% identical to glycine betaine ABC transporter periplasmic binding protein ProX (Escherichia coli K-12 substr. MG1655)
RXN-8638 [EC: 7.6.2.9]; 7.4.2.- [EC: 7.6.2.9]

Predicted SEED Role

"L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (339 amino acids)

>GFF482 Glycine betaine/L-proline transport substrate-binding protein ProX (TC 3.A.1.12.1) (Variovorax sp. SCN45)
MTKKTSLIARGLLALAFVACGATALATGNDLPGKGVTVLPLKSSLAEEAFQTLLVMRALE
KLGYTVEPMKDLEPATEHLAIANGDATFMTNHWSLLHADFYKNSGGDAKLWRKGVYSDGA
VQGYLIDRKTAEQYGITSIAQLKDPAIAKLFDADGDGKADLTGCNPGWGCELAIENHLTA
YQLRDTVTHKQGSYAALMADTIARFKQEKPVLYYTWTPYWVSAVLRPGADVVWLQVPFSS
SQGDAGNVDTQLPNGKNYGFQANQEQIVANRAFVEKNPAAGKLFEVMKLPISDINAQNLR
MSQGANTQQDVERHTDGWIKAHQELFDGWIAQARAAAAP