Protein Info for GFF4819 in Sphingobium sp. HT1-2

Annotation: amylo-alpha-1,6-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 739 PF14742: GDE_N_bis" amino acids 42 to 235 (194 residues), 199.1 bits, see alignment E=9.4e-63 PF22422: MGH1-like_GH" amino acids 320 to 625 (306 residues), 71.2 bits, see alignment E=1.8e-23 PF06202: GDE_C" amino acids 381 to 665 (285 residues), 26.7 bits, see alignment E=4e-10

Best Hits

KEGG orthology group: None (inferred from 76% identity to sjp:SJA_C1-25870)

Predicted SEED Role

"amylo-alpha-1,6-glucosidase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (739 amino acids)

>GFF4819 amylo-alpha-1,6-glucosidase (Sphingobium sp. HT1-2)
MSLTQPQPGVPGIAGGQALSQAQTQFFIPATASLHERRPRTLKHGDSFAVFDHSGDAISG
PGSPEGFYHRDTRHLSHLYLTVNGMRPILLSSALRDDNAMLTCDLANPDFLGSDGRVEVE
HDLIHLRRSRFLWEGAIYERIIVRNFDIAERRVSVGLSFASDFADLFEIRGMTRLRRGTM
HEPKVEEADVLLGYKGRDGLERSTHLHFDPAPAQLTAEQALFDVSVAPGGRCTLFARVAC
EQMSEVDQPLPKLFLKSLREAMRALQVSRRRSASMRSSNDLFDAVTRRSVSDISMLSTGT
EYGPYPYAGIPWFSTVFGRDALITALQTIWMDPAISRGVLGYLAAHQATHFDPAADAEPG
KILHEVRHGEMAELGEVPFRYYYGSIDSTPLFVMLAGAYLERTGDLQFLAGILPNIESAL
QWIDNHGDRDGDGFVEYGRLTDQGLQNQGWKDSHDSIFHADGSIAKGPIALAEVQAYVYG
AWQAASSIYRATGDLDRSEAYLARAVDLQDRFDATFFDAELGTYVLALDGQKQPCRIRSS
NAGHVLYTGLARTERAPMVVATLMAPASFSGWGVRTIASTERRYNPMSYHNGSIWPHDNA
LIAAGFSRYGFQRQAAQIFEGLFAAATYVDLLRLPELFCGFPRRRSQGPTFYPVACSPQA
WSAAAPLSLIQASLGLGFNVSSNEIIFRQPTLPTFLDSLFIASLPLGAGEADIMLDRLGD
TAVVKPQHRDSRVRIVTIA