Protein Info for PS417_24600 in Pseudomonas simiae WCS417

Annotation: hydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 97% identity to pfs:PFLU5399)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)" (EC 2.7.4.7)

MetaCyc Pathways

superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
hydroxymethylpyrimidine salvage (2/2 steps found)
thiamine diphosphate salvage II (4/5 steps found)
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
thiamine diphosphate salvage IV (yeast) (3/7 steps found)
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)

KEGG Metabolic Maps

Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.49 or 2.7.4.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U711 at UniProt or InterPro

Protein Sequence (265 amino acids)

>PS417_24600 hydrogenase (Pseudomonas simiae WCS417)
MNIYSSRPVVLCLSGHDPSGGAGLQADIEALLAQGCHAAPTVTALTVQNTVNVSDFRVLD
REWVLAQANAVLGDSEVAAVKLGMLGSTAMVDTVVELLQAHPHLPVVCDPVLRAGGGGSL
GKDDVGYAMRERLLPLSLIATPNLPEARILAELPEGTADECAEKLLPYIKHLLITGGHGD
EHEVHNRLYSRDGTRQTFTCQRLPGSYHGSGCTLASALSGRIAQGEGLVSAVQSALNYTW
RTLRDAEQLGQGQFVPLRLPLDFCS