Protein Info for GFF4807 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Fumarylacetoacetase (EC 3.7.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 38% identical to Y643_PYRHO: Uncharacterized protein PH0643 (PH0643) from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

KEGG orthology group: None (inferred from 82% identity to pol:Bpro_2124)

MetaCyc: 52% identical to 3-fumarylpyruvate hydrolase (Bradyrhizobium sp. JS329)
RXN-10445 [EC: 3.7.1.20]

Predicted SEED Role

"Fumarylacetoacetase (EC 3.7.1.2)" in subsystem Homogentisate pathway of aromatic compound degradation or Salicylate and gentisate catabolism (EC 3.7.1.2)

MetaCyc Pathways

L-tyrosine degradation I (4/5 steps found)
gentisate degradation I (2/3 steps found)
3-phenylpropanoate degradation (5/10 steps found)
5-nitroanthranilate degradation (2/6 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.7.1.2

Use Curated BLAST to search for 3.7.1.2 or 3.7.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (241 amino acids)

>GFF4807 Fumarylacetoacetase (EC 3.7.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSNPSSAMPDTTYLFAPPATVSVPVVGRAERFPVHRIYCVGRNYEEHAKEMGFTGREPPF
FFLKPADAIVVADAGQTADMPYPSLTGNLHHEIELVVAIGVGGRNIKAADAAKHIYGYAV
GLDMTRRDLQNDMKKQGRPWCIGKAYDQSAPIGPITPAAQAGQVNEAPIWLQVNGADRQR
STVAKLIWNVAETIEHLSAAWELQPGDLIYTGTPEGVAAVVKGDTLHGGVDGLTPISVRV
A