Protein Info for GFF4804 in Variovorax sp. SCN45

Annotation: tRNA threonylcarbamoyladenosine biosynthesis protein TsaE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 PF02367: TsaE" amino acids 38 to 166 (129 residues), 117.2 bits, see alignment E=2.5e-38 TIGR00150: tRNA threonylcarbamoyl adenosine modification protein YjeE" amino acids 39 to 171 (133 residues), 111 bits, see alignment E=2e-36

Best Hits

KEGG orthology group: K06925, UPF0079 ATP-binding protein (inferred from 91% identity to vpe:Varpa_3971)

Predicted SEED Role

"TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (185 amino acids)

>GFF4804 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (Variovorax sp. SCN45)
LGESEFPGQSLNTNNEWMADDHLPIVETQTSTRILRWRSEDDTAAFAQALAASPALRDAF
IALHGDLGAGKTTFVRHLLRALGIAGRIKSPTYAVVEPHDAPDGLQIFHFDFYRFADPRE
WDDAGFRDIFAGPGLKLAEWPENAAGRTPIADLAIKIEAMTDDTRSVTLLANTPRGSDLL
ARIAA