Protein Info for GFF4802 in Xanthobacter sp. DMC5

Annotation: Metal reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 PF00724: Oxidored_FMN" amino acids 9 to 352 (344 residues), 184.3 bits, see alignment E=1.4e-57 PF07992: Pyr_redox_2" amino acids 404 to 500 (97 residues), 41.4 bits, see alignment E=4.4e-14 PF00070: Pyr_redox" amino acids 404 to 438 (35 residues), 22.2 bits, see alignment (E = 6e-08) PF01266: DAO" amino acids 404 to 470 (67 residues), 31.6 bits, see alignment E=4.7e-11 PF00890: FAD_binding_2" amino acids 405 to 440 (36 residues), 22.9 bits, see alignment 1.7e-08 PF12831: FAD_oxidored" amino acids 405 to 447 (43 residues), 34.6 bits, see alignment 4.8e-12 PF13450: NAD_binding_8" amino acids 407 to 440 (34 residues), 33.2 bits, see alignment (E = 1.8e-11)

Best Hits

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (677 amino acids)

>GFF4802 Metal reductase (Xanthobacter sp. DMC5)
MTPAPYERLLSPLALGHVTLRNRIAMMPHAVMFGGGYGSAIDRTIAYHVERAKGGAGLIV
MSNFLMPASWRRLATWGEALETSPLGGLDLANDASLQPHYRRMIEAIKGEGACFVSQLNI
SGRQLRSPGTTQYGVPLLAPSPLPCPRTGEIPKEMAEADIAEYVATMVEAALNLKAAGAD
GVELFAAQGYLLHEFLSPATNKRADRYGGPLENRMRFLLEGLAAIRAAVGPDFLIGARIN
GRDDVPRGMTPQDAIAIAVRLKDEGISYLNVSGLTALHYPGWIADITTPEAQFAQEAGAI
RSATAGLPVGVSSRIATPKAAEAVLAAGEADFIGMARALISDPELPLKAARGDAKAIRVC
TYSNQSCIVGLDRGRGVGCIHNPAVGREAQIGIGRMRPAQRIKRVAVVGGGPAGLASARI
AAERGHSVTLFERTTRLGGQNLMTSAVASRHGFAEIHRWQIEMLARLHVDIRLSIAADRA
LIEREGFDALVVATGSAPRRDGYTSLRPGADGIAGADLPHVHTVWDVFEAPEGFIGPVIV
VEDDPHLSGTAAAEMLAARGHNVTIVTPHMHAGADLPVHHAPALYRRLAGLRIQVVPATF
VTAIRPGSLDCEARFGGVERQIAHDGPVVLAMGNRADDALARAFVGSGIELHVVGDAVAP
RQVDMAILDGERAGWML