Protein Info for PS417_24545 in Pseudomonas simiae WCS417

Annotation: isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01261: AP_endonuc_2
  • amino acids 21 to 247 (227 residues), 146.2 bits, E=7.3e-47

Best Hits

Swiss-Prot: 37% identical to OTNI_HAEIN: 2-oxo-tetronate isomerase (otnI) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 82% identity to pba:PSEBR_a895)

MetaCyc: 37% identical to 2-dehydrotetronate isomerase (Haemophilus influenzae Rd KW20)
RXN-18592 [EC: 5.3.1.35]; 5.3.1.35 [EC: 5.3.1.35]

Predicted SEED Role

"Hydroxypyruvate isomerase (EC 5.3.1.22)" (EC 5.3.1.22)

MetaCyc Pathways

• D-erythronate degradation II (1/4 steps found)
• L-threonate degradation (1/4 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.22

Use Curated BLAST to search for 5.3.1.22 or 5.3.1.35

Sequence Analysis Tools

See A0A1N7UCI4 at UniProt or InterPro

Protein Sequence (250 amino acids)

>PS417_24545 isomerase (Pseudomonas simiae WCS417)
MLKFNAHLGFQFNELPFLQRIEAAAAAGFRAVEFPSPYEFDASLLADHLAQYNLPLIQFA
APAGVTKGIAALQGKEQEFRDGLREAARYAKALNCSDVHIMSGVTTPQDESAPAYVSNLE
YAVKYLEDQGLRALIEVICPQAMPGYYMSDFSKAQQVLETFTSVGLILDLYHAQLLTGDA
AGVLAQFYDRTVHVQIADNPGRHEPGTGTMDFAALFAALEQRGYRGWIGCEYRPSGTTVE
SLEWLKPLRA