Protein Info for GFF4791 in Sphingobium sp. HT1-2

Annotation: Domain often clustered or fused with uracil-DNA glycosylase / Uracil-DNA glycosylase, putative family 6 (EC 3.2.2.27)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR03915: probable DNA metabolism protein" amino acids 9 to 239 (231 residues), 211.5 bits, see alignment E=2.4e-66 PF13566: DUF4130" amino acids 81 to 238 (158 residues), 129.4 bits, see alignment E=1.1e-41 TIGR03914: uracil-DNA glycosylase family domain" amino acids 241 to 468 (228 residues), 345.2 bits, see alignment E=2.7e-107 TIGR00758: uracil-DNA glycosylase, family 4" amino acids 292 to 458 (167 residues), 133.7 bits, see alignment E=8.1e-43 PF03167: UDG" amino acids 306 to 465 (160 residues), 117.7 bits, see alignment E=4.6e-38

Best Hits

KEGG orthology group: K02334, DNA polymerase bacteriophage-type [EC: 2.7.7.7] (inferred from 74% identity to sch:Sphch_2436)

Predicted SEED Role

"Domain often clustered or fused with uracil-DNA glycosylase / Uracil-DNA glycosylase, putative family 6"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>GFF4791 Domain often clustered or fused with uracil-DNA glycosylase / Uracil-DNA glycosylase, putative family 6 (EC 3.2.2.27) (Sphingobium sp. HT1-2)
MHRVTLAGPDDFEGWRDAARALALAAIPADTILWDVAGSAAPDLFGSDATPLPPAPADAA
FSVPRAFVDLAQSAILHRDPARFALLYSLLLAVRAVPGRMEDRADPLLRRVEDLAKTVRR
DIHKMRAFVRFRELDEGDGPRFIAWFEPDHHIVRANAGFFTRRFASMRWSILTPEVSIHW
DGEKLTEGPGAQRGDAPAEDPVEDVWKGYYAAIFNPARLKTGAMLSEMPRKYWRNLPEAA
LIPDLVAGAQAREAAMIATAPRPASRPTARTAPRTGNSRAAWQALRDEAMACTRCPLHGP
ATQTVFGEGPLDASLLFIGEQPGDQEDLAGRPFVGPAGQLFDAALAEAGVDRARAYVTNA
VKHFKYEQRGKRRLHQTPDVPEIRACRWWLEQELDLIRPRIIVALGATAARALLGKPVTI
SQMRGRPLPLDDGAEAWITVHPSYLLRLPDPDRQAQEQARFVADLRMVGERLHALT