Protein Info for GFF4786 in Xanthobacter sp. DMC5
Annotation: putative acyl-CoA dehydrogenase fadE25
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 42% identical to ACDA_BACSU: Acyl-CoA dehydrogenase (acdA) from Bacillus subtilis (strain 168)
KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 70% identity to bbr:BB4752)MetaCyc: 36% identical to cyclohexane-1-carbonyl-CoA dehydrogenase monomer (Syntrophus aciditrophicus)
R282-RXN [EC: 1.3.8.11]
Predicted SEED Role
"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4); Butyryl-CoA dehydrogenase (EC 1.3.99.2)" (EC 1.3.8.4, EC 1.3.99.2)
MetaCyc Pathways
- L-leucine degradation I (5/6 steps found)
- cyclohexane-1-carboxylate degradation (anaerobic) (1/2 steps found)
- benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
- crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Brassinosteroid biosynthesis
- Butanoate metabolism
- Caprolactam degradation
- Fatty acid metabolism
- Geraniol degradation
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.-, 1.3.99.2
Use Curated BLAST to search for 1.3.8.11 or 1.3.8.4 or 1.3.99.- or 1.3.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (380 amino acids)
>GFF4786 putative acyl-CoA dehydrogenase fadE25 (Xanthobacter sp. DMC5) VSGWLNEDQIQVQDLVRRVARERVAPRAQEIDLTAEYPHDMFELIRELGLFTLPFAPEYG GTGSMLSACVAVEELGRVCYNTAYLLVVQWVPIGAILAGGTPEQKQKYLPGLASGNLRGA LSLTEPQSGSDVAGIRTRAKRDGQGWRLSGSKIWCTNSGMADFVLVAARTGEDDQRGKIN LFVVEKGTPGFEVGRKEDKIGARGVPSHALFFDNCYLPAEAMLGDEETGFRVAMAALNDS RPIIGARGVGLAQGAIDHTIEFIKSRRAFGQAVSDFQGVRWMIADMVIQTQASRNLVYEA AAAVDAGVTGKALAQLSAISKCHATDTAMKVATDAVQLFGAAGVSNEYPINRYFRDAKVL QIIEGTNQIQRNIVARNVLD