Protein Info for GFF4776 in Xanthobacter sp. DMC5

Annotation: 2-haloacrylate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 transmembrane" amino acids 120 to 138 (19 residues), see Phobius details amino acids 244 to 260 (17 residues), see Phobius details PF08240: ADH_N" amino acids 31 to 115 (85 residues), 63.6 bits, see alignment E=2.8e-21 PF00107: ADH_zinc_N" amino acids 156 to 273 (118 residues), 73.9 bits, see alignment E=1.8e-24 PF13602: ADH_zinc_N_2" amino acids 215 to 330 (116 residues), 58.6 bits, see alignment E=2e-19

Best Hits

KEGG orthology group: K00344, NADPH2:quinone reductase [EC: 1.6.5.5] (inferred from 43% identity to fri:FraEuI1c_5387)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.5

Use Curated BLAST to search for 1.6.5.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (334 amino acids)

>GFF4776 2-haloacrylate reductase (Xanthobacter sp. DMC5)
MIPNSMKALVATDYRGPASVRIAQLPVPAPGAGEVLIRAGASALNFLDLLMLEGKYQVKP
PIPFVPGRDLAGEVVAVGAGVTGLAVGQRVAAQPAFGAFAEYAVAPDYACQPLPDLIDDV
VGAASGIVVATVVGALALRGRVARGQRVLITGAAGGVGTAAIQYATELGAVPVALVSSAA
KAEATLRLGATVSVRSDLLSSGKGALRDALKGEGIDDVDVVVDMVGGPNVDGLLRCLKPG
GRFVIVGFASGHIPMIPANYLLLKDIEVHGSSLDRLFRTRDPDLANGIREAFNLLETGRM
KAAVDVVLPFEDFAEGARRLAARETVGKVVFKGF