Protein Info for PS417_24395 in Pseudomonas simiae WCS417

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 transmembrane" amino acids 27 to 48 (22 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 24 to 441 (418 residues), 411 bits, see alignment E=3e-127 PF00529: CusB_dom_1" amino acids 35 to 396 (362 residues), 59.3 bits, see alignment E=6e-20 PF13533: Biotin_lipoyl_2" amino acids 66 to 110 (45 residues), 33.8 bits, see alignment 4.6e-12 PF13437: HlyD_3" amino acids 293 to 407 (115 residues), 59.3 bits, see alignment E=1.1e-19

Best Hits

Swiss-Prot: 52% identical to PRTE_DICCH: Proteases secretion protein PrtE (prtE) from Dickeya chrysanthemi

KEGG orthology group: K12537, protease secretion protein HasE (inferred from 95% identity to pfs:PFLU5357)

Predicted SEED Role

"ABC exporter for hemopore HasA, membrane fusion protein (MFP) family component HasE" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1TY08 at UniProt or InterPro

Protein Sequence (441 amino acids)

>PS417_24395 hemolysin D (Pseudomonas simiae WCS417)
MNQSKPVLVSEAPNNVLALDDKQYSRLGWLLVLGGFAGFLGWAALAPLDKGVAVPGKVMV
SGHRKTVQHPAGGIVERIDVRDGDVVSAGQVLLRLKETPLRAQMQSLRSQYLASLASEAR
LSAESEGLSAIRFGPELLNDPEAAGTLSLQRQLFNSRAQALATEQQGLRETIAGAEAQLR
GTRDSQASKVHQRAALNEQLQGLRELAREGYIPRNRLLDSERLYSQIDGAIAEDYGRIGQ
LQRQVMELRLRIRQLGEDFQKDLRGQLAETRTRSDDLRNRLASAEFELANSLVRAPASGV
VVGLDVYTEGGVIKPGQALMDIVPQGEPLLVEARVPVQMVDKVHPGLPVELMFSAFNQST
TPRVAGEVTLVSADRQVDERTDEPYYTLRAQVSAAGMQQLDGVQIRPGMPVETFVKTGER
SMLNYLFKPLMDRTHMALVEE