Protein Info for GFF4763 in Variovorax sp. SCN45

Annotation: NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 7 to 478 (472 residues), 433.2 bits, E=7.1e-134
• PF00361: Proton_antipo_M
  • amino acids 128 to 428 (301 residues), 252.4 bits, E=2.8e-79

Best Hits

Swiss-Prot: 74% identical to NUON_POLNA: NADH-quinone oxidoreductase subunit N (nuoN) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 93% identity to vpe:Varpa_4012)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (495 amino acids)

>GFF4763 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) (Variovorax sp. SCN45)
MIDKISWVTIYPEIVLLVMTCIIALVDLSDTSPRRTRTYVLTLLTLAVVAVLSGLQAYDA
KTAYGFGGMVVSDPMGNWLKCFATVALMVTLVYGRPYAADREMLRGGELFTVSMFALLGM
FVMISGSNFLLIYLGLELLTLSSYALVALRRDNAVASEAAMKYFVLGAMASGFLLYGLSM
MYGATGSLDVNEVFKTIASGKVNHQVLVFGLVFIVAGLAFKVGAAPFHMWVPDVYQGAPT
AITLLIGAAPELAAFAIIIRLLVDALQPLAIDWQQMLAVLAIASLLVGNLAAIAQSNLKR
MLAYSTISQMGFMLLGLVAGSDQQGTYNAQYAYSASMFYIVTYVLTTLASFGIILLLARE
GFESEEITDLAGLNQRSPLYAGVMAIAMFSLAGLPPLVGFYAKLAVLQALIASGHAIYIG
LAVFAVMMSLIGAFYYLRVVKVMYFDAPVTATTVSAPRDVRAVLTINGALILILGIVPGG
LMTLCYDAIVATLAH