Protein Info for GFF4761 in Variovorax sp. SCN45

Annotation: NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 40 to 61 (22 residues), see Phobius details amino acids 72 to 90 (19 residues), see Phobius details amino acids 95 to 114 (20 residues), see Phobius details amino acids 126 to 143 (18 residues), see Phobius details amino acids 149 to 168 (20 residues), see Phobius details amino acids 188 to 207 (20 residues), see Phobius details amino acids 225 to 243 (19 residues), see Phobius details amino acids 264 to 282 (19 residues), see Phobius details amino acids 294 to 315 (22 residues), see Phobius details amino acids 322 to 348 (27 residues), see Phobius details amino acids 351 to 371 (21 residues), see Phobius details amino acids 389 to 410 (22 residues), see Phobius details amino acids 430 to 452 (23 residues), see Phobius details amino acids 491 to 512 (22 residues), see Phobius details amino acids 550 to 574 (25 residues), see Phobius details amino acids 654 to 674 (21 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 12 to 672 (661 residues), 813.3 bits, see alignment E=1e-248 PF00662: Proton_antipo_N" amino acids 77 to 127 (51 residues), 79.3 bits, see alignment 1.6e-26 PF00361: Proton_antipo_M" amino acids 143 to 431 (289 residues), 266.2 bits, see alignment E=3.4e-83

Best Hits

Swiss-Prot: 64% identical to NUOL_NEIMB: NADH-quinone oxidoreductase subunit L (nuoL) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 93% identity to vap:Vapar_3499)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (677 amino acids)

>GFF4761 NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3) (Variovorax sp. SCN45)
MSATLSASTLLAVPLAPLVGAAVAGLFGTKFGGNHIGRKVTHSLTILGVLVAFIISAMTL
KSVIVDGARFNATLYEWMVVGGLKMEVGFMVDGLTAMMMCVVTFVSLMVHIYTIGYMEED
DGYNRFFAYISLFTFSMLMLVMSNNMLQLFFGWEAVGLVSYLLIGFWFNKPTAIFANMKA
FLVNRVGDFGFILGIGLIAAYAGTLNYTEAFAKAGTLAGITFPGTEWMLITVICICLFIG
AMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLFELSDTALSFILV
IGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVALGASAYSVAVFHLMTHAFFKALL
FLGAGSVIIGMHHNQDIRWMGGVRKYMPITWITSLLGSLALIGTPFFAGFYSKDSIIEAV
HESHLWGANFAYYAVLAGVFITAFYSFRMYFLVFHGKERYDQNPDAHHDDHGHGHDDHGH
GHDHKPHESPWVVWLPLVLLAIPSVVIGFMTIEPMLFGEFFKNAIFVDGAKHHAMKELEE
GFHGPVAMAIHGLTTAPFWLALAGVVLSWYMYMINPALPAAIKRAFGPIYRLLENKYYMD
WFNENVIARATRALGTGLWKGGDQALIDGAVVNGSWKLVGRISGVVRWMQSGYIYHYAFA
MLLGIFILMTYFVWFKR