Protein Info for GFF4760 in Sphingobium sp. HT1-2

Annotation: tRNA-modifying protein YgfZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 PF01571: GCV_T" amino acids 10 to 81 (72 residues), 27.7 bits, see alignment E=7.9e-11 TIGR03317: folate-binding protein YgfZ" amino acids 133 to 197 (65 residues), 73.8 bits, see alignment E=4.1e-25

Best Hits

KEGG orthology group: K06980, (no description) (inferred from 82% identity to sch:Sphch_0288)

Predicted SEED Role

"Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (245 amino acids)

>GFF4760 tRNA-modifying protein YgfZ (Sphingobium sp. HT1-2)
MTDTTLTDRAVLRISGEEARPFLQGLLTRDVLTLKDGEARWTALLTPQGKALFDFILWGD
HGDILIDCEAAQADALAKRLTIYRLRRKVVIARDESLAVHWAIDAADKPRDPRLPELGAR
WLAPASDGDASAAFRAHRLSLGVFEGAAELGQDQTLWLETNAEELHGVDYDKGCYVGQEN
TARMHYRNKVNRRLVAVPLEQAEEKRQRAALPDLGLSIELQRVEAIDPATLPAWLATAIA
AQAAE