Protein Info for GFF4756 in Variovorax sp. SCN45

Annotation: NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 PF13510: Fer2_4" amino acids 1 to 76 (76 residues), 70.4 bits, see alignment E=3.2e-23 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 631 (628 residues), 668.5 bits, see alignment E=5.7e-205 PF00111: Fer2" amino acids 5 to 65 (61 residues), 27.6 bits, see alignment 7e-10 PF10588: NADH-G_4Fe-4S_3" amino acids 84 to 120 (37 residues), 59.9 bits, see alignment 4.2e-20 PF22117: Fer4_Nqo3" amino acids 136 to 203 (68 residues), 109.6 bits, see alignment E=2e-35 PF22151: Fer4_NDSU1" amino acids 220 to 265 (46 residues), 50.2 bits, see alignment 4.5e-17 PF00384: Molybdopterin" amino acids 273 to 633 (361 residues), 167.4 bits, see alignment E=1.4e-52

Best Hits

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 94% identity to vap:Vapar_3504)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (723 amino acids)

>GFF4756 NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3) (Variovorax sp. SCN45)
MIEIELDGKKVEVTEGSMVMHAADKAGTYIPHFCYHKKLSIAANCRMCLVDVEKAPKPMP
ACATPVTQGMIVRTKSEKAIKAQQSVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS
SRYEEEKRVVFHKDVGPLISMEEMSRCIHCTRCVRFGQEVAGVMELGMSHRGEHSEIETF
VGDSVDSELSGNMIDICPVGALTSKPFRYSARTWELSRRKSVSPHDSTGANLIVQVKNNH
VMRVVPLENEDVNECWIADRDRFSYEALNGTERLTQPMLKQGGQWQQVDWQTALEYVANG
LKQIKADHGAQSIGTLVSPHSTLEELQLATMLTRELGSDNIDYRLRNAEFAAFEGVRWLG
TSIASLTQVQRALVVGSNLRKEHPLFAQRIRQAVRKGAALSVITSASLMADRNAWAIDVA
QSSIVEADQWVEALAAVAAAIGETNGAAAPLAPKNAPDAAAKAIAASLLSGERKAVLLGN
AAAHHAQASSLLALANWIGAQTGATVGYLTEAANTVGAQLVGAFPKNGGLDAGRMLAAGS
GLKAVLLLNNEPVFDSAAGKAAADVIGNAQMVVTLSPFKANLEFSDVLLPIAPFTETPGT
FVNAEGRLQGFHAVVKPQGETRPAWKVLRVLANLLGLPGFAFESTAEVLATIGDKGAVPA
NALSNATAAAAVASNGAAAAPVVASIYQLDSIVRRAPSLQLTADARNASAGSNVTARAEE
ALA