Protein Info for GFF4731 in Variovorax sp. SCN45

Annotation: Assimilatory nitrate reductase large subunit (EC 1.7.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 PF04879: Molybdop_Fe4S4" amino acids 1 to 50 (50 residues), 65.4 bits, see alignment 7.3e-22 PF00384: Molybdopterin" amino acids 62 to 490 (429 residues), 248.1 bits, see alignment E=2.9e-77 PF01568: Molydop_binding" amino acids 588 to 707 (120 residues), 88.6 bits, see alignment E=5.8e-29 PF04324: Fer2_BFD" amino acids 868 to 917 (50 residues), 26.1 bits, see alignment 1.7e-09

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 89% identity to vpe:Varpa_4045)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (930 amino acids)

>GFF4731 Assimilatory nitrate reductase large subunit (EC 1.7.99.4) (Variovorax sp. SCN45)
MRETRSTCPYCGVGCGVIIESHGDEITGVRGDPEHPANFGRLCTKGSTLHLTASATVTRQ
TRLLQPMQRIVRREAPTVVTWDTALDNAAGKFAQVIRDHGPDSVGFYVSGQLLTEDYYVF
NKLAKGLIGTNNIDTNSRLCMSSAVAGYKQTLGADAPPACYDDLKHAQCILIVGSNTAWA
HPILFRRLEDAKAANPALKIVVADPRRTDTAEIADLFLPIQPGTDVMLFNGMLHLMLWEG
WMDAKYIAAHTNGFDALKATVRECTPDKVAQVCGVSKEDLLEAARLFATSPATLSLYCQG
LNQSSSGTAKNAALINLHLATAQIGKPGAGPFSLTGQPNAMGGREVGGLANLLSAHRDLA
NPDHRAEVAALWGVPSVPEKPGKTAVEMFQAAADGEIRALWIACTNPAQSMPDQATVRRA
LERAEFVVVQEAFATTATCAFADLLLPATTWGEKEGTVTNSERRISRVRPAVPAPGAARN
DWSIAVAFAHRLERQMGRAFTLFPYETAESVWNEHRESTRGRDLDITGMSYAMLETAGPQ
QWPLKEGESTGRARLYEDGVFPTPDGRARFVDTIYKPVAEAREARYPFSLNTGRLRDQWH
GMSRTGTAGRLFGHVPEPVVQMNAQDMARRQIREGDLVHLTSRRGSIMLPARASAEVGLS
QAFVAMHWGEEYLSGCSSNGTRLAGINALTTPAFCPTSKQPELKHTPVKILKAELPWSLL
AMAWLPSDAALTAQRALQPLMALFPFATCVPFAGNTQGAERSGILFRAAAHDAPDDALVE
KIEALLGLQTSDALRYADRRRGQRRSARLVRRADGSAGLEAFLLGGDTSAEAWIATLLRE
ELPAQSYGRLLLSPGSKAPVAVQSRGKTVCTCFNVTDLAIETELGRCGGSPDERLAALQG
ALKCGTNCGSCVPELKRRVREHPAAQSLAA