Protein Info for GFF473 in Sphingobium sp. HT1-2

Annotation: Chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 632 TIGR02350: chaperone protein DnaK" amino acids 3 to 598 (596 residues), 981.3 bits, see alignment E=7.9e-300 PF00012: HSP70" amino acids 4 to 599 (596 residues), 935.2 bits, see alignment E=1.4e-285 PF06723: MreB_Mbl" amino acids 132 to 372 (241 residues), 57.7 bits, see alignment E=9.3e-20

Best Hits

Swiss-Prot: 89% identical to DNAK_SPHWW: Chaperone protein DnaK (dnaK) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 94% identity to sch:Sphch_2944)

MetaCyc: 64% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (632 amino acids)

>GFF473 Chaperone protein DnaK (Sphingobium sp. HT1-2)
MAKVIGIDLGTTNSCIAVMDGGKPKVIENAEGARTTPSIVAFAKDGERLIGQPAKRQAVT
NPDNTIFAVKRLIGRRFDDPMTKKDMELVPYDIAKGPNGDAWVKAGGEEYSPSQISAFIL
QKMKETAESYLGETVTQAVITVPAYFNDAQRQATKDAGQIAGLEVLRIINEPTAAALAYG
LDKQDGKTIAVYDLGGGTFDISILEIGDGVFEVKSTNGDTFLGGEDFDAKLVEYLAADFK
KAESIDLTKDKLALQRLKEAAEKAKIELSSAQSTEVNLPFITADQNGPKHLVKTITRADL
ERLVADLIKRTMEPCKKALADAGVTASEISEVVLVGGMTRMPKVREAVKEFFGKEPHTGV
NPDEVVAMGAAIQAGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRMIDRNTTIPAKKSQV
YSTADDNQQAVTIRVFQGEREMAADNKLLGQFDLVGIPPAPRGVPQIEVTFDIDANGLVN
VSAKDKGTGKEQQIRIQASGGLSDSDIDQMVKDAERFAEEDKKRREAAEAKNNAESLVHT
TEAQLAEHGDKVDASLKGEIEAAIAETKSAIEGGDAEAMKAKAQELATVAMKLGQAIYEK
EQAAAASPEAEAPKADDGVVDAEFSEVDDNKA