Protein Info for GFF4714 in Sphingobium sp. HT1-2

Annotation: Oleate hydratase (EC 4.2.1.53)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 transmembrane" amino acids 24 to 42 (19 residues), see Phobius details PF06100: MCRA" amino acids 22 to 520 (499 residues), 701.1 bits, see alignment E=4.5e-215

Best Hits

Swiss-Prot: 54% identical to OLHYD_MACCJ: Oleate hydratase (MCCL_0076) from Macrococcus caseolyticus (strain JCSC5402)

KEGG orthology group: K10254, myosin-crossreactive antigen (inferred from 92% identity to rpc:RPC_3601)

Predicted SEED Role

"Oleate hydratase (EC 4.2.1.53)" (EC 4.2.1.53)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.53

Use Curated BLAST to search for 4.2.1.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (588 amino acids)

>GFF4714 Oleate hydratase (EC 4.2.1.53) (Sphingobium sp. HT1-2)
MHYSSGNYEAFVRPRKPEGADKKTAWFVGSGLAGLAGAAFLIRDGGVSGDRITILEELEI
PGGALDGLDVPEKGFVIRGGREMEEHFECLWDLYRSIPSLEIEDASVLDEFYRLNKDDPN
FSLQRTTQNQGQDVPDKALLTLNDKAQKDLLSVFLATREEMENKRINEVFGEDFLKSNFW
LYWRTMFAFEEWHSALEMKLYLHRFIHHIGSLADFSSLKFNRYNQYESMVLPLVKWLTDH
GVTFRYGVEVTDVDFDIQPGRKQATRIHWKERGVEGGVDLGPDDLVFITIGSLTENSDNG
DHRTPAKLNEGPAPAWDLWRRIAAKDPAFGRPDVFGAHIPETKWASASITALDARIPAYV
EKITKRNPFTGKIVSAGIVTVKDSAWLLSWTVHRQPHFKKQPKDQMIAWFYALFVDCPGD
FVKKPMQECTGEEITQEWLYHLGVPVEDIPELAASGASTVPTMMPYITAFFMPRQAGDRP
DVVPEGAVNFAFIGQFAESKQRDCIFTTEYSVRTPMEAVYTLMNVERGVPEVFNSTYDIR
TLLAAIGPLRDGKGIDLPGPAFLRKLLMKKLEGTEIAKLLEEFHLIEE