Protein Info for GFF4713 in Sphingobium sp. HT1-2

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 829 transmembrane" amino acids 174 to 195 (22 residues), see Phobius details amino acids 206 to 226 (21 residues), see Phobius details amino acids 238 to 261 (24 residues), see Phobius details amino acids 277 to 295 (19 residues), see Phobius details amino acids 431 to 453 (23 residues), see Phobius details amino acids 459 to 482 (24 residues), see Phobius details amino acids 775 to 794 (20 residues), see Phobius details amino acids 800 to 821 (22 residues), see Phobius details PF04945: YHS" amino acids 38 to 81 (44 residues), 55.5 bits, see alignment 1.4e-18 PF19335: HMBD" amino acids 129 to 154 (26 residues), 56.3 bits, see alignment (E = 7.5e-19) TIGR01511: copper-translocating P-type ATPase" amino acids 221 to 825 (605 residues), 579.4 bits, see alignment E=1.8e-177 TIGR01525: heavy metal translocating P-type ATPase" amino acids 240 to 824 (585 residues), 616.4 bits, see alignment E=1.1e-188 TIGR01494: HAD ATPase, P-type, family IC" amino acids 285 to 793 (509 residues), 299 bits, see alignment E=1e-92 PF00122: E1-E2_ATPase" amino acids 314 to 494 (181 residues), 190.8 bits, see alignment E=4.8e-60 PF00702: Hydrolase" amino acids 512 to 729 (218 residues), 135.8 bits, see alignment E=7.5e-43 PF12710: HAD" amino acids 649 to 725 (77 residues), 29.1 bits, see alignment E=3.9e-10 PF08282: Hydrolase_3" amino acids 701 to 758 (58 residues), 21 bits, see alignment 7.2e-08

Best Hits

Swiss-Prot: 70% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 80% identity to sjp:SJA_C1-04830)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (829 amino acids)

>GFF4713 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Sphingobium sp. HT1-2)
MADDDQKSERAAEVTGAHGHHHHHEPDGETPSGHVDHDPVCGMVVDPARTAHKAEYEGER
YAFCSAGCKAKFDADPVHYAARVAHAEPHHEGAATHGDHSHPSHTHRLDRHEHHPVAALT
AAVTEGTIWTCPMHPEIRRDAPGSCPICGMALEPLIAAADAGPSPELADMTRRFWIGLAL
ALPVFLLEMGGHLIPALHHLVPMHISIWIQFALATPVVLWAGWPFFARGWASIVNRSLNM
FTLIAMGTGVAWGYSIVATFAPQLFPQAFRSADGTVAVYYEAAAVITVLVLLGQVMELRA
REQTSGAIRALLNLAPKTARRIGRDDDEEDIPVEAVGIGDRLRVRPGETVPVDGVVEQGR
SSIDESMVTGESMPVTRVVGEAVVGGTLNQTGALVIRAEKVGRDTMLARIVQMVADAQRS
RAPIQRMADQVSGWFVPVVIAIALLAFAAWGLWGPEPRLSHGLIAAVSVLIIACPCALGL
ATPMSIMVGIGKGAAAGVLIKNAEALERMEKVDTLVVDKTGTLTQGKPAVTRIVATPGFV
EETILRLAASVEMASEHPLARAIVAAAEERKIAIPDVADFDSPTGKGAIGRVGGRHVVLG
STTFLAEHGVDTAPLAAQADELRRDGATAIYIGIDKAIGGIFAIADTIKATTPEALKALH
AEGIRIVMLTGDNRTTAEAVARKLGIDEVEADVLPDQKAAVVERLKAQGKVVAMAGDGVN
DAPALAAADVGIAMGSGTDVAIESAGVTLLKGDLTGIVRARRLSQATMANIRQNLAFAFI
YNAAGVPIAAGALYPTFGLLLSPIIAAAAMALSSVSVIGNSLRLRTVKA