Protein Info for GFF4696 in Xanthobacter sp. DMC5

Annotation: Formate dehydrogenase-O major subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 1 to 805 (805 residues), 1116.8 bits, see alignment E=0 PF00384: Molybdopterin" amino acids 1 to 381 (381 residues), 71.8 bits, see alignment E=5.8e-24 PF01568: Molydop_binding" amino acids 684 to 800 (117 residues), 52.9 bits, see alignment E=3.6e-18

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 95% identity to xau:Xaut_0662)

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (812 amino acids)

>GFF4696 Formate dehydrogenase-O major subunit (Xanthobacter sp. DMC5)
MTNSWTDIKNTDLVIIMGGNAAEAHPCGFKWVTEAKATRGAKLVVVDPRFTRSAAVADYY
APIRQGTDIAFLLGVINYCIQNDKIQWDYVKAFTNVGYIVKEGFGYQDGLFTGYDEMKRD
YNRSSWDYEIGPDGYAVVDDTLQHPRCVWNLLKQHVSVYTPELVERITGTPKDKFLKVAS
MIAECSSPTKTMTSMYALGWTQHSKGSQNIRCMAMLQLLLGNIGVRGGGMNALRGHSNIQ
GLTDIGLMSNLIPGYLTIPTEKEPDFNAYMSTRGFKPLRPNQMSYWQNYKKFFVSFQKAM
WGASATADNNWAYDYLPKLDVPSYDILRAFELMKQGKMNIYFCQGFNPLQAFPNKAKLAE
ALAKLKLLVIMDPLETETARFWENHGVYNQADPSKIQTEVIQLPTTCFAEDNGSLVNSGR
WLQWHWAAGTPPGEAKTDIYIMAQIHLRLKEMYKKDGGAFPDPIVNLTWNYKDPHEPLPD
ELAKELNGYVVSDVLDPNDPTKKVLEAGKQVPTFGVLRDDGSTAAGCWIYSGCYTEAGNM
MARRDNSDPDDTGAYLKWSFAWPANRRIIYNRASCNIDGKPWDDTRKLIWWDGAKWTGYD
VPDIAPTAKPQDVGPFIMNPEGVSRLFTRGMMRDGPFPVHYEPFESPVANLIAPKVRGNP
VARVFKDDFAQFADVASKEFPYAATSYRLTEHFHYWTKNNHVNSVLQPEFFVEISEQLAK
EKSIQNAGWVRVWSKRGSVFAKAYVTKRIRPLMVDGKPVHIVGIPIHWGFVGAAKKGFPA
NVLTPFVGDANIETPEFKAFLVNIEPSTGPVA