Protein Info for GFF4683 in Variovorax sp. SCN45

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 771 transmembrane" amino acids 154 to 174 (21 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details amino acids 212 to 228 (17 residues), see Phobius details amino acids 234 to 252 (19 residues), see Phobius details amino acids 382 to 405 (24 residues), see Phobius details amino acids 416 to 441 (26 residues), see Phobius details amino acids 719 to 740 (22 residues), see Phobius details amino acids 746 to 765 (20 residues), see Phobius details PF00403: HMA" amino acids 71 to 129 (59 residues), 26.3 bits, see alignment 1.2e-09 TIGR01511: copper-translocating P-type ATPase" amino acids 196 to 766 (571 residues), 404.2 bits, see alignment E=2.2e-124 TIGR01525: heavy metal translocating P-type ATPase" amino acids 214 to 766 (553 residues), 595.1 bits, see alignment E=3.4e-182 TIGR01494: HAD ATPase, P-type, family IC" amino acids 237 to 748 (512 residues), 275 bits, see alignment E=1.9e-85 PF00122: E1-E2_ATPase" amino acids 265 to 446 (182 residues), 180.6 bits, see alignment E=3.4e-57 PF00702: Hydrolase" amino acids 463 to 677 (215 residues), 115 bits, see alignment E=8.8e-37

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 85% identity to vpe:Varpa_4094)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (771 amino acids)

>GFF4683 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Variovorax sp. SCN45)
MTNENALTRPVPHAHSHPHSHGDDHGHEHAKPHAHSHAEDSCCGSSSSCGAIPLAPAGAD
GADVPKGALLFRIPTMDCAVEESEIRRALEPVAGVKALRFRLGERTMAITTEDGALPEAL
AAIRKAGFKPEPLNPGGTQAAAAPAQIAGMNMGLVRLIAALVLAIAAESISFMALDGMGF
KAVEVVLALGAIGLAGLDTYKKGFAALVRGRLNINALMAVAVTGAFIIGQWPEAAMVMAL
YAIAELIEARAVDRARNAIQSLLALAPEQAEVKQADGSWKTVMANAVALGDVARIRPGER
VPLDGIVTEGTSAIDQAPVTGESIPVDKTIGDPVFAGTINQTAALEFRVTAVASNTTLAR
IIHAVEEAQGSRAPTQRFVDKFAAIYTPTVFVLALAVAVLTPLFMDWAWMQSVYKALVLL
VIACPCALVISTPVTVVSALASAARRGILIKGGTYLEEARKLKAIALDKTGTITEGKPKL
VESVLVDASGSEAAVFAVAASIAGRSDHPVSKAIAEGLKSPRQEVADFTALPGRGVGATL
AGQAYVLGNHRLIEERGLCSPALEAELKRHEEAGRTVTLLASDKAVLALFAVADTIKESS
QAAVAELRALGVTPVMLTGDNTATAKTIGAHAGIDDVRGNLLPEEKLDAIKAMQQRYGAA
AMTGDGINDAPALAQADIGFAMGGAGTDTAMEAADVVIMNDDLRRIPETIRLSRRAHSVL
WQNITLALGIKGVFFVLAVFGSATMWMAVFADMGASLLVVANGLRLMRVPK