Protein Info for GFF4681 in Xanthobacter sp. DMC5

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 26 to 1110 (1085 residues), 1465.7 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 157 to 363 (207 residues), 238.2 bits, E=2.1e-74
  • amino acids 713 to 935 (223 residues), 92.9 bits, E=6.3e-30
• PF02787: CPSase_L_D3
  • amino acids 458 to 535 (78 residues), 88.1 bits, 9.1e-29
• PF02142: MGS
  • amino acids 1016 to 1101 (86 residues), 79.1 bits, 6.8e-26

Best Hits

Swiss-Prot: 76% identical to CARB_BRUME: Carbamoyl-phosphate synthase large chain (carB) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• allantoin degradation IV (anaerobic) (6/9 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1148 amino acids)

>GFF4681 Carbamoyl-phosphate synthase large chain (Xanthobacter sp. DMC5)
METDPVVLAGSAARVAAVESSKTDMPKRTDIETILIIGAGPIVIGQACEFDYSGTQAVKA
LKEEGYRVVLVNSNPATIMTDPDMADATYIEPITAEIVTKIIEKERGDRSKGFALLPTMG
GQTALNCALSLKKMGTLEKYDVEMIGATAEAIDKAEDRELFREAMTKIGLSTPKSRQIKT
LPQALEALDEVGLPAIIRPSFTMGGTGGGIAYNKAEFIEIIERGIDASPTNEVLVEESVL
GWKEYEMEVVRDKADNCIIICSIENIDPMGVHTGDSITVAPALTLTDKEYQIMRDASLAV
LREIGVETGGSNVQFAVNPEDGRLIVIEMNPRVSRSSALASKATGFPIAKVAARLAVGYT
LDEIENDITGGATPASFEPTIDYVVTKIPRFAFEKFPGADPVLTTAMKSVGEAMAIGRTF
QESLQKALRSLETGLSGLDDIEIEGLGQGDDKNAVRAALGTPTPDRLLYVAQAMRLGLSD
EQIHSACKIDPWFLAEIRGIVEMEQKVRRLGLPETAGQFRRLKAMGFSDARLATLAGLHE
GEVRARRQALDVRPVYKRIDTCAAEFASPTAYMYSTYETPFAGVLADEAQPSEREKVIIL
GGGPNRIGQGIEFDYCCCHAAFALKDAGYEAIMVNCNPETVSTDYDTSDRLYFEPLTAED
VLELIAREKTRGTLKGVIVQFGGQTPLKLAKALEDADVPILGTSPDAIDLAEDRDRFKTL
LDRLKIRQPANGISYSVEQARLVAAELGYPMVVRPSYVLGGRAMQIIREESQLGDYLLET
LPGLVPQEIKARYPNDKTGQINTVLGKNPLLFDRYLSDAIEVDVDALCDGKDVFIAGIME
HIEEAGIHSGDSACTLPPYSLSPDTLAALEKQTAAMALALGVGGLMNVQYAIKGDEIYVL
EVNPRASRTVPFVAKVIGLPIAKIAARVMAGEKLASFNLVQPKLGHIAVKEAVFPFARFP
GVDTVLGPEMRSTGEVMGLDTDFGVAFAKSQLGGGTKVPKSGTVFVSLKDGDKIRILPTM
KMLVDLGFKIIATSGTQRFLEEHGVPATKINKVLEGRPHIVDAIKNGGVQLVFNTTEGAQ
ALADSRSLRRAALLQKVPYYTTLSGAIAAAQGIKAYVGGDLTVRPLQGYFGAEGDSAGVS
PRGAASVA