Protein Info for GFF468 in Xanthobacter sp. DMC5

Annotation: Lipopolysaccharide export system ATP-binding protein LptB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 263 PF00005: ABC_tran" amino acids 27 to 186 (160 residues), 113.4 bits, see alignment E=2e-36 PF12399: BCA_ABC_TP_C" amino acids 235 to 260 (26 residues), 40 bits, see alignment (E = 3.3e-14)

Best Hits

Swiss-Prot: 44% identical to BRAF_PSEAE: High-affinity branched-chain amino acid transport ATP-binding protein BraF (braF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 56% identity to axy:AXYL_05220)

MetaCyc: 42% identical to branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG (Escherichia coli K-12 substr. MG1655)
ABC-15-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (263 amino acids)

>GFF468 Lipopolysaccharide export system ATP-binding protein LptB (Xanthobacter sp. DMC5)
MNTPRSTGDLLEVEGLTCRFAGVTAVDDLSFRVKAGEIKAVIGPNGAGKSTLFNMIAGVT
RPTDGAIKFDGARVDRLPTHARARRGIARTFQNLQIFREMSVLENVMVGRHLRGRATTLS
ALLHIPSVGAEERAMEATAMALLERFGLAAKAGLPAGSLAFGQMKVLELARALASEPRLL
LLDEPAAGLPHAEADKMAETIRDLNRDGMTVLLVEHNMRMVMSLSHDILVLNNGRRIAEG
TAEDVRRHPEVLSAYLGEEAVDA