Protein Info for PS417_02390 in Pseudomonas simiae WCS417

Annotation: DNA topoisomerase IV subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 8 to 629 (622 residues), 1028.5 bits, see alignment E=0 PF02518: HATPase_c" amino acids 32 to 136 (105 residues), 52.7 bits, see alignment E=1.1e-17 PF00204: DNA_gyraseB" amino acids 222 to 390 (169 residues), 142.2 bits, see alignment E=2.4e-45 PF01751: Toprim" amino acids 417 to 526 (110 residues), 51.5 bits, see alignment E=1.9e-17 PF00986: DNA_gyraseB_C" amino acids 562 to 625 (64 residues), 81.6 bits, see alignment E=7.2e-27

Best Hits

Swiss-Prot: 90% identical to PARE_PSEAE: DNA topoisomerase 4 subunit B (parE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 96% identity to pfs:PFLU0499)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1SNV2 at UniProt or InterPro

Protein Sequence (635 amino acids)

>PS417_02390 DNA topoisomerase IV subunit B (Pseudomonas simiae WCS417)
MATPSASSYNADAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAKSVH
VILHADHSLEVSDDGRGMPVDIHPEEGVPGVELILTKLHAGGKFSNKNYQFSGGLHGVGI
SVVNALSTLVRVKVKRDGNEYQMTFADGYKATDLEVIGTVGKRNTGTSVYFAPDPKYFDS
PKFSISRLKHVLKAKAVLCPGLVVTFEDKGTGEKVEWHYEDGLRSYLEDSVSDFERLPNE
PFCGSLAGNKEAVDWALLWLPEGGDSVQESYVNLIPTAQGGTHVNGLRQGLLDAMREFCE
YRNLLPRGVKLAPEDVWERIAFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSL
WLNEHPELGLALAELAINNAGRRLKASKKVERKRITQGPALPGKLADCAGQDPMRSELFL
VEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQEVHNIAVAIGVDPGAAD
MSQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVEAGHVYVAMPPLYRIDLGKEIY
YALDEAERDGILDRLVAEKKRGKPQVTRFKGLGEMNPPQLRETTMDPNTRRLVQLTLGDD
FAATSEMMDMLLAKKRAPDRKSWLESKGNLAEVLG