Protein Info for GFF4653 in Xanthobacter sp. DMC5

Annotation: putative GST-like protein YibF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 199 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF13417: GST_N_3" amino acids 3 to 75 (73 residues), 63.3 bits, see alignment E=5.6e-21 PF02798: GST_N" amino acids 7 to 71 (65 residues), 40.2 bits, see alignment E=9e-14 PF13409: GST_N_2" amino acids 8 to 72 (65 residues), 59.1 bits, see alignment E=1.4e-19 PF00043: GST_C" amino acids 100 to 187 (88 residues), 22.7 bits, see alignment E=2.5e-08 PF13410: GST_C_2" amino acids 123 to 184 (62 residues), 46.6 bits, see alignment E=7.6e-16

Best Hits

Swiss-Prot: 38% identical to YIBF_ECOLI: Uncharacterized GST-like protein YibF (yibF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 76% identity to xau:Xaut_2729)

Predicted SEED Role

"Glutathione S-transferase family protein" in subsystem Glutathione: Non-redox reactions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (199 amino acids)

>GFF4653 putative GST-like protein YibF (Xanthobacter sp. DMC5)
MLLRHSPTSPFVRKVRVAAAILGIAVELEPADTLDANDTLRQQNPLGKIPALLTGDGEVL
FDSSVILAYLDHLAGDGRILPTEPVARIKVLRREALVDGMMDAAILILYEARFRPQEKHE
ARWLEHQAGKRLRALDALEAEVEPYSGTPNVGDIALACALGWFDFRFAGDWRADHPKLVA
WFDAFSAAHPQFADSTPVA