Protein Info for GFF4651 in Xanthobacter sp. DMC5
Annotation: Putrescine--pyruvate aminotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to SPUC_PSEAE: Putrescine--pyruvate aminotransferase (spuC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K12256, putrescine aminotransferase [EC: 2.6.1.-] (inferred from 94% identity to xau:Xaut_2727)MetaCyc: 56% identical to putrescine--pyruvate aminotransferase (Pseudomonas aeruginosa)
RXN-8 [EC: 2.6.1.113]
Predicted SEED Role
"Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18)" in subsystem Pyruvate Alanine Serine Interconversions (EC 2.6.1.18)
MetaCyc Pathways
- superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
- β-alanine degradation II (2/2 steps found)
- putrescine degradation V (2/2 steps found)
- β-alanine biosynthesis II (4/6 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Aminophosphonate metabolism
- Arginine and proline metabolism
- Caprolactam degradation
- Lysine biosynthesis
- Lysine degradation
- Methionine metabolism
- Nucleotide sugars metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.-, 2.6.1.18
Use Curated BLAST to search for 2.6.1.- or 2.6.1.113 or 2.6.1.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (477 amino acids)
>GFF4651 Putrescine--pyruvate aminotransferase (Xanthobacter sp. DMC5) VTSAPLSNSTAHWRALDAAHHLHPFSDTKALNAEGVRVITRAEGVYVFDSDGNRILDGMS GLWCSQVGHGRHAIVDAIAQQLHQLDYYNTFFKTTHPAAAELAAAISEVAPAHMNHVFFT SGGSEAVDTVLRMVRHYWASLGKPEKHIFISRRNGYHGSTMGGASLGGMKPMHAQGGLPI PGIIHVAQPYWWAEGGEMSREDFGLYAAREVARAIDEAGPENVAAFIGEPIQGAGGVIIP PANYWPEVEKICRERDVLLICDEVICGFGRTGRWFGAEFMGVKPDLITFAKGVTSGYFPL GGVIVGDRFAEGFIEHGGDFNHGYTYSGHPGGCAAALANLKIMHEEKLVDRVRTDIGPYL QERWLKLAEHPLVGEARMVGLIGAMELTPDKSTRAAFAAEEGTVGLICRDFSFKEGLVMR AVRDGMILAPPFTLSHEEADELVATAWRVLDLTHAELIKRGMMVPVPRVPARAIASV