Protein Info for GFF4645 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Cobyrinic acid A,C-diamide synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to COBB_PSEU5: Hydrogenobyrinate a,c-diamide synthase (cobB) from Pseudomonas stutzeri (strain A1501)
KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 65% identity to vap:Vapar_2548)Predicted SEED Role
"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (10/13 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.-, 6.3.5.9
Use Curated BLAST to search for 6.3.1.- or 6.3.5.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (399 amino acids)
>GFF4645 Cobyrinic acid A,C-diamide synthase (Hydrogenophaga sp. GW460-11-11-14-LB1) MWLALATDHPVHNLDLWMTGELDIRTRLHAAAQEADLIIVEGVMGLFDGEPSAADLARRF GLHVLAVIDASAMAGTFGALAFGLQHFRPGLPWAGVLANRVASGRHAQLLQDALPSQDGE PTSSWMGAVMRNPSFALPERHLGLTVASELLDAMARLDAAADALLNTPLGKLDSADLARW SVSFDAPTERPSSIAPLLAGKTVAVARDAAFCFIYPANLDTLRALGAQVVFFSPLANHPV PACDAVWLPGGYPELHAETLSISDTTRDSLVVHVNSGRPVWAECGGMMVLFDAIVDLEGR SHVSWGLLSGVVRMQPRLAALGPQKLELARGKLRGHTFHYSVCETALEPVLHAVSARGDA KGAGEAVYQRGALRASYFHTWMASSPTAAAALFLPKVLA