Protein Info for PS417_23725 in Pseudomonas simiae WCS417

Annotation: methylmalonate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 12 to 488 (477 residues), 748.2 bits, E=1.9e-229
• PF00171: Aldedh
  • amino acids 20 to 484 (465 residues), 486.1 bits, E=4.7e-150

Best Hits

Swiss-Prot: 60% identical to MMSA_ANOGA: Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (AGAP002499) from Anopheles gambiae

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 100% identity to pfs:PFLU5203)

MetaCyc: 71% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• myo-inositol degradation I (7/7 steps found)
• β-alanine degradation II (2/2 steps found)
• L-valine degradation I (6/8 steps found)
• myo-, chiro- and scyllo-inositol degradation (7/10 steps found)
• β-alanine degradation I (1/2 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• 2,4-dinitrotoluene degradation (3/7 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Sequence Analysis Tools

See A0A1N7U6J8 at UniProt or InterPro

Protein Sequence (505 amino acids)

>PS417_23725 methylmalonate-semialdehyde dehydrogenase (Pseudomonas simiae WCS417)
MNASADISVQQVKLLINGEWVESKTTEWQDIVNPATQEVLAKVPFATADEVNAAIDAAHR
AFQTWKLTPIGARMRIMLKLQALIREHSKRIAVVLSNEQGKTIADAEGDIFRGLEVVEHA
CSIGTLQMGEFSENVAGGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIACGNTFVL
KPSEQDPLSTMLLVELALEAGVPAGVLNVVHGGKDVVDALCTHKDIKAVSFVGSTAVGTH
VYDLAGKHGKRVQSMMGAKNHAVVLPDANREHTLNALVGAGFGAAGQRCMATSVVVLVGA
SKQWLPDLKALAQKLKVNAGSEAGTDVGPVISKRAKARILELIESGVKEGAKLELDGRDI
KVPGFEQGNFVGPTLFSGVTPSMQIYTQEIFGPVLVVLEVATLDEAIALVNANPFGNGTG
LFTQSGAAARKFQSEIDVGQVGINIPIPVPVPFFSFTGSRGSKLGDLGPYGKQVVQFYTQ
TKTVTSRWFDDDTVNDGVNTTINLR