Protein Info for GFF4633 in Sphingobium sp. HT1-2

Annotation: Broad-specificity glycerol dehydrogenase (EC 1.1.99.22), subunit SldA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 797 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 41 to 57 (17 residues), see Phobius details amino acids 64 to 80 (17 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 120 to 139 (20 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 14 to 797 (784 residues), 959.4 bits, see alignment E=5.9e-293 PF13360: PQQ_2" amino acids 226 to 385 (160 residues), 27.2 bits, see alignment E=4.3e-10 amino acids 675 to 767 (93 residues), 27.8 bits, see alignment E=2.9e-10 PF01011: PQQ" amino acids 227 to 255 (29 residues), 20.2 bits, see alignment (E = 5.8e-08)

Best Hits

Swiss-Prot: 55% identical to SLDA_GLUTH: Glycerol dehydrogenase large subunit (sldA) from Gluconobacter thailandicus

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 73% identity to pzu:PHZ_c1591)

MetaCyc: 55% identical to D-sorbitol dehydrogenase large subunit (Gluconobacter oxydans IFO 3255)
D-sorbitol dehydrogenase (acceptor). [EC: 1.1.99.21]

Predicted SEED Role

"Broad-specificity glycerol dehydrogenase (EC 1.1.99.22), subunit SldA" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2 or 1.1.99.21 or 1.1.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (797 amino acids)

>GFF4633 Broad-specificity glycerol dehydrogenase (EC 1.1.99.22), subunit SldA (Sphingobium sp. HT1-2)
MTNAPPRRSIAALILGIVFGLIGLVLTGGGAWLLISGGSPYYLVTGIALIVAGVLLARGR
KVGGWLYIVIFAVTLLWAYWEVGLNPWALVPRVVAPLVLLIALQLVWPTLDRSAGRWRRA
WVGVAVTFAVTIGFFWSIAGNFIPPVLQGLPDARYAMADPSIGTTGADWPAYGGTNSARR
YSSLAQITPANVGNLKRAWLIHTGDLPASAQIRNTYGAENTPLKIGDTLYVCTPKSMVIA
LDPATGKQKWRFDPKVSDEAIPYTAACRGVSYYAVPGATADAPCAQRIILGTLDARIMAL
DARTGEQCHDFGNNGQVRITDGMGQVPPGYVSITSPPTIVRGVIVTGHQVLDGQDRWAPS
GVIQAYDAVTGKMRWAWDMMHPERSGPPPAGQTYARGTPNMWTIASGDEQLGLVYLPMGN
SAADYYSSLRRPEENRYATSLVAIDVMTGRPRWNFQAVRKDVWDYDFGAQATLIDFPTAR
GPVPAMLLPSKQGDIYVLDRRTGRPLTPVGDIKAPQIGVEPAQRAATQRISLYHTLRKPD
LTEKDMWGMSPIDQMICRIQFRRASYKGFFTPPEADKRSIEYPGYNGGTDWGGIAVDPAR
GVIVANYNDMPNYVRLVPRKEANKKGWAPRDQARGEIGGAEGAGDPQAHTPYAIDVNAGW
RLPFTGLLCKQPPYGGIRAIDIATGKTIWDRPFGTARTNGPFGIPSMLPLTIGTPNNGGS
VVTAGGLIFVAAATDNLIRAIDLKTGKTLWQDVLPAGGQATPMTYSQNGKQYLVIMAGGH
HFMQTPVGDALIAYALN