Protein Info for PS417_23685 in Pseudomonas simiae WCS417
Updated annotation (from data): anthranilate 1,2-dioxygenase (deaminating, decarboxylating) large subunit [EC: 1.14.12.1]
Rationale: Specifically important for: L-Tryptophan. anthranilate is an intermediate in the breakdown of tryptophan (via kynurenine) (KEGG_correct)
Original annotation: anthranilate 1,2-dioxygenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to ANTDA_ACIAD: Anthranilate 1,2-dioxygenase large subunit (antA) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
KEGG orthology group: K05599, anthranilate 1,2-dioxygenase (deaminating, decarboxylating) large subunit [EC: 1.14.12.1] (inferred from 96% identity to pfs:PFLU5194)MetaCyc: 75% identical to anthranilate dioxygenase oxygenase component large subunit (Acinetobacter)
Anthranilate 1,2-dioxygenase (deaminating, decarboxylating). [EC: 1.14.12.1]
Predicted SEED Role
"Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)" in subsystem Benzoate degradation (EC 1.14.12.10)
MetaCyc Pathways
- anthranilate degradation I (aerobic) (1/1 steps found)
- benzoate degradation I (aerobic) (1/2 steps found)
- 2-nitrobenzoate degradation II (1/3 steps found)
- mandelate degradation to acetyl-CoA (9/18 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
- meta cleavage pathway of aromatic compounds (3/10 steps found)
- toluene degradation IV (aerobic) (via catechol) (4/13 steps found)
- superpathway of aerobic toluene degradation (12/30 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (15/42 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Carbazole degradation
- Fluorobenzoate degradation
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.14.12.1
Use Curated BLAST to search for 1.14.12.1 or 1.14.12.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7URJ9 at UniProt or InterPro
Protein Sequence (463 amino acids)
>PS417_23685 anthranilate 1,2-dioxygenase (deaminating, decarboxylating) large subunit [EC: 1.14.12.1] (Pseudomonas simiae WCS417) MSGARSVEQWKTFIEGCLDFRPADEVFRIARDMFTEPQLFDLEMELIFEKNWIYACHESE LANNHDFVTMRAGRQPMIITRDGEGQLNALINACQHRGTTLTRVGKGNQSTFTCPFHAWC YKSDGRLVKVKAPGEYPDGFDKATRGLKKARIESYKGFVFISLDVAGTDSLEDFLGDAKV FFDMMVAQSATGELEVLPGKSAYTYDGNWKLQNENGLDGYHVSTVHYNYVATVQHRQQVN TENGAGAGSTLDYSKLGAGDANTDDGWFAFNNGHSVLFSDMPNPSVRSGYATIMPRLVEE HGQQKAEWMMHRLRNLNIYPSLFFLDQISSQLRIIRPVAWNKTEIISQCLGVKGESDADR ENRIRQFEDFFNVSGMGTPDDLVEFREAQRGFQGRLERWSDISRGSHRWETGPTPNSEAI GIQPAMTGTEFTHEGLYVNQHRNWQQFLLNGLDRQALTLREVK