Protein Info for PS417_02355 in Pseudomonas simiae WCS417

Annotation: thiamine biosynthesis protein ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 630 transmembrane" amino acids 474 to 492 (19 residues), see Phobius details PF13667: ThiC-associated" amino acids 25 to 102 (78 residues), 107 bits, see alignment E=3.5e-35 TIGR00190: phosphomethylpyrimidine synthase" amino acids 141 to 593 (453 residues), 717.5 bits, see alignment E=2.4e-220 PF01964: ThiC_Rad_SAM" amino acids 142 to 591 (450 residues), 617.9 bits, see alignment E=8.7e-190

Best Hits

Swiss-Prot: 96% identical to THIC_PSESM: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 99% identity to pfs:PFLU0492)

MetaCyc: 72% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U3X8 at UniProt or InterPro

Protein Sequence (630 amino acids)

>PS417_02355 thiamine biosynthesis protein ThiC (Pseudomonas simiae WCS417)
MKSTKLKNTVHLSESAKVDSGSVQPFTRSQKIYVQGTRPDIRVPMREISLDVTPTDFGGE
INAPVTVYDTSGPYTDPNVIIDVRKGLADVRSPWIEVRNDTERLPGLSSHFGQQRLSDAE
LTALRFAHVKNPRRAKAGANVTQMHYARKGIITAEMEYVAIRENMKLEEARATGLLDQQH
AGHSFGASVPKIITPEFVREEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSAL
GSSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGG
AAEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLA
HHKENFTYTHFDEICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKTAWK
HDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMI
GWFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARF
EFRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQR
IEAVDVDVAKGLAEQAQRFKQEGSQLYKKV