Protein Info for GFF4600 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 28 to 220 (193 residues), 189.2 bits, E=8.8e-60
• PF08659: KR
  • amino acids 29 to 194 (166 residues), 62.6 bits, E=6.8e-21
• PF13561: adh_short_C2
  • amino acids 37 to 272 (236 residues), 180.3 bits, E=7e-57

Best Hits

Swiss-Prot: 32% identical to Y325_THEMA: Uncharacterized oxidoreductase TM_0325 (TM_0325) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

KEGG orthology group: K00046, gluconate 5-dehydrogenase [EC: 1.1.1.69] (inferred from 85% identity to rfr:Rfer_2493)

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (17/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin biosynthesis I (13/15 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (25/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (25/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (25/34 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• L-idonate degradation (2/3 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• ketogluconate metabolism (5/8 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100, 1.1.1.69

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.69

Protein Sequence (277 amino acids)

>GFF4600 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTDRAFLHHPEDDNMTRTIPQLFDLKGKTALITGGSRGLGLQMAHALGEAGARVLVSSRK
AEDLESATAELQAAGIDARWVAADCSKEEDIHRLADESLQRLGDIDILVNNAGAAWGAPA
EDHPVEAWDKVMNLNVRSYFILSQVIARRSMIARKSGRIINTASIAGLGGNPSGMNTIAY
NTSKGAVINFTRALAAEWGKYGITVNAICPGFFPSKMTVGTLKTMGEEKLASHAPLRRLG
DDEDLKGLTVLYASDAGKHITGQWLAVDGGVSVITGG