Protein Info for GFF4586 in Sphingobium sp. HT1-2

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 828 transmembrane" amino acids 190 to 206 (17 residues), see Phobius details amino acids 212 to 233 (22 residues), see Phobius details amino acids 245 to 263 (19 residues), see Phobius details amino acids 268 to 285 (18 residues), see Phobius details amino acids 434 to 453 (20 residues), see Phobius details amino acids 465 to 487 (23 residues), see Phobius details amino acids 778 to 797 (20 residues), see Phobius details amino acids 803 to 821 (19 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 247 to 819 (573 residues), 568.8 bits, see alignment E=2.4e-174 TIGR01494: HAD ATPase, P-type, family IC" amino acids 270 to 794 (525 residues), 251 bits, see alignment E=2.7e-78 PF00122: E1-E2_ATPase" amino acids 297 to 493 (197 residues), 183.8 bits, see alignment E=2.3e-58 PF00702: Hydrolase" amino acids 511 to 730 (220 residues), 119.8 bits, see alignment E=2.1e-38

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 73% identity to npp:PP1Y_AT22779)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (828 amino acids)

>GFF4586 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Sphingobium sp. HT1-2)
MIQKLRLDVPLLLPEVTDTADGCVSRLISLIESRPGVSEVHVTKEPAGTLPQLCIHYDPD
AISLPRIRELAKAAGTQIAERIGHAVWEVDGIGHQRRARSIGETLRALPGVLEAEAILPG
TVRVEFDRTITSEDGILTGLRDAGVTPKALAASGKKPVAHDHDKDHDHPAVEKHDHDHGH
GGLLGPNTELIFALACGGALGVGYLIETFVTAPAWVPFALFIAAYVFGGFYTVREAIENL
RQKRFEIDTLMLVAAAGAAALGAWAEGALLLFLFSLGHALEHYAMGRAKRAIEALAELAP
RTATVRRMDGGSSVVPVEELVLGDVVIIKPDERIAADGFVIKGNSAVNQAPVTGESIPVD
KEPVESADSARANPDRVAAASRVFAGTINGSGLIEIEVTRLSNESTLAKVVKMVSEAETQ
KSPTQRFTDRFERFFVPAVLVLAFLLLFAWVVVDEPFRDSFYRAMAVLVAASPCALAIAT
PSAVLSGVARAARGGVLVKGGAPLELLGSLDAIAFDKTGTLTMGEPRIQQIIPAPGVTRE
ELMALAVAVESLSDHPLAQAIARDGREHIGDHPVPEAENLKSLTGRGISALVGEDEVLIG
KAEMFRSDGIAPLSTEMDAAIKTLREAGQTSMVVRSGSRDMGAIGLLDTPREAARAALER
LREIGIKRMIMISGDHQRVADAIGKQVGIDEAWGDLMPEDKVEAIKKLRAETKVAMVGDG
VNDAPAMATATVGIAMGAAGSDVALETADVALMADNLSHLPFAVGLSRSTRWVIRQNVFV
SLGVVAFLVPATILGLGIGPAVALHEGSTLLVVINALRLLAYRDPVRA