Protein Info for GFF4579 in Sphingobium sp. HT1-2

Annotation: CzcABC family efflux RND transporter, outer membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 420 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF02321: OEP" amino acids 36 to 216 (181 residues), 59.8 bits, see alignment E=1.7e-20 amino acids 248 to 412 (165 residues), 78 bits, see alignment E=4.4e-26

Best Hits

KEGG orthology group: None (inferred from 69% identity to sch:Sphch_4104)

Predicted SEED Role

"Heavy metal RND efflux outer membrane protein, CzcC family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (420 amino acids)

>GFF4579 CzcABC family efflux RND transporter, outer membrane protein (Sphingobium sp. HT1-2)
MFRSIAALLAAVSCVAVAQAQEMPAAVAGASPYTLDQAVAAAGGSAPAAEAAQANVEAAR
AARTVAGLRPNPTVQTQVENIAGSGPYRGVGSAESTVSMAVPIELGGKRSARIAVANAQT
DKALLVSAITQADIRLQITQLYIEAAAAERRVQTARDQARIAGDAANAASVRVKAGRASP
IEEQRANVAKINADAELVRTIRLAQASRANLERRIGQPMGGPLDTAWLERLPRGYGPEVP
PSAVGTLALAAADADLAVADANVRLANSQRVPNLEAGPGLRRLSATNDTALIFTVTMPIP
IFNSGKAAVAQARAQRNQVEAQKRMTALDVEQAITDAQTSASNAAVTAQTATGPALEAAQ
EAARIARIGYREGKFGQLDLLDAERTLAQTRLAAIDALASYQNARAQLERLTAPAPEQGN