Protein Info for GFF4566 in Sphingobium sp. HT1-2

Annotation: Methylphosphotriester-DNA--protein-cysteine S-methyltransferase (EC 2.1.1.n11) / ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 PF02805: Ada_Zn_binding" amino acids 20 to 83 (64 residues), 96.7 bits, see alignment E=1.6e-31 PF00165: HTH_AraC" amino acids 107 to 137 (31 residues), 32.6 bits, see alignment (E = 1.7e-11) PF12833: HTH_18" amino acids 111 to 188 (78 residues), 65.5 bits, see alignment E=1.1e-21 PF02870: Methyltransf_1N" amino acids 198 to 239 (42 residues), 21.4 bits, see alignment 7.4e-08 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 271 to 350 (80 residues), 112.1 bits, see alignment E=4.9e-37 PF01035: DNA_binding_1" amino acids 272 to 351 (80 residues), 115.6 bits, see alignment E=1.8e-37

Best Hits

Swiss-Prot: 46% identical to ADA_ECOLI: Bifunctional transcriptional activator/DNA repair enzyme Ada (ada) from Escherichia coli (strain K12)

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 76% identity to sch:Sphch_0072)

MetaCyc: 46% identical to DNA-binding transcriptional dual regulator / DNA repair protein Ada (Escherichia coli K-12 substr. MG1655)
2.1.1.M37 [EC: 2.1.1.M37]; Methylated-DNA--[protein]-cysteine S-methyltransferase. [EC: 2.1.1.M37, 2.1.1.63]; 2.1.1.63 [EC: 2.1.1.M37, 2.1.1.63]

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63 or 2.1.1.M37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (362 amino acids)

>GFF4566 Methylphosphotriester-DNA--protein-cysteine S-methyltransferase (EC 2.1.1.n11) / ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (Sphingobium sp. HT1-2)
MNQMTRPEPVPAAMPDAADCWTAFLARDRRQDGCFVGAVLTTGIYCKPSCPARHPKRENM
IFLPDGAAARAAGFRACLRCRPDEVGRDQQAVEAAIRLIRTAETPPRLEEMADHVGYAPH
HFHRLFKRITGLTPAAYARDLRTERLKAALATPGSVTDAIYEAGYNAPSRAYADAERHLG
MTPSAWREGGRGVDIRHVVIGTSLGALLVAATERGLCRIAFEEGEADLRRRYPNATLLPA
DAALDRWARAVAALVDDPGAGADLPTDVGGTAFQQAVWAALRAIPAGETRSYGEIAAAIG
RPGAVRAAGTACGDNGLAILVPCHRVLRSDGSLGGYAYGVDRKRNLLERERRAKMTAKAT
GE