Protein Info for GFF4564 in Sphingobium sp. HT1-2

Annotation: Putative protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 301 to 322 (22 residues), see Phobius details amino acids 330 to 350 (21 residues), see Phobius details PF13365: Trypsin_2" amino acids 57 to 205 (149 residues), 80.6 bits, see alignment E=2e-26 PF00089: Trypsin" amino acids 58 to 225 (168 residues), 49.6 bits, see alignment E=4.8e-17

Best Hits

KEGG orthology group: None (inferred from 78% identity to sjp:SJA_C1-26800)

Predicted SEED Role

"putative protease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (519 amino acids)

>GFF4564 Putative protease (Sphingobium sp. HT1-2)
MVALFRRLRPIAALVASLVLLSPARAEQQDITAAARSVVRVALVATNGADAYFVGHGSGI
AIAPDKVLTNAHVVELAREEKDLVIGVIPSEGKKSYGGRIIAYSPGNDLALIQLEEGSLP
VATFYAGAVDDGQHVTAIGYPGTVDRAQGLSLKQLVEPLGTVKTSGSISSGRSSQSFDTI
LHTAPLAAGNSGGPLVDDCGRVLGVNSFGSISDGNDAEFGFAVSWREVASFLRQAGVSSL
HTVVACRTMAEADAAEASITQRESQLTEQNDRAAADKREQALQQARDTAERDVISGRENA
MAGAAVLLALAVLGLGAGGLFYSQGREKRATWFIAGGGILLMGALGLFVFKPSFASIDER
VKLPEDQSVVSNKAFAWAGDNICRIDLNRSRLTVSQPNDVGLNWAEGGCVNGDTQYQATG
TTWQRAHVPDEGNYVSRSEFDPTTGLLRVQRWLPDGDTMDKARALLKDGPIKGCSSDSTM
LTRIATLQSDLAALLPPQPNERLVYHCQKGRLAPADPAP