Protein Info for PS417_23345 in Pseudomonas simiae WCS417

Annotation: amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00497: SBP_bac_3" amino acids 38 to 256 (219 residues), 57.1 bits, see alignment E=8.1e-20

Best Hits

Swiss-Prot: 72% identical to YHDW_ECO57: Putative amino-acid ABC transporter-binding protein YhdW (yhdW) from Escherichia coli O157:H7

KEGG orthology group: K09969, general L-amino acid transport system substrate-binding protein (inferred from 97% identity to pfs:PFLU5128)

Predicted SEED Role

"Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UH48 at UniProt or InterPro

Protein Sequence (342 amino acids)

>PS417_23345 amino acid ABC transporter substrate-binding protein (Pseudomonas simiae WCS417)
MNPFKTTLAAIGATAVLGLASTAEAGTTLDAIQKKGFIQCGVSDGLPGFGVPDSKGKMTG
IDVDVCHAVAAAVFGDASKVKFTQLTAKERFTALQSGEIDLISRNTTWTSSRDSGMGLVF
TGVTYYDGIGFLVNNKLGVTAAKQLDGATVCIQAGTTTELNVSDYFRTHGMKYTPITFDT
ADESAKGLEAGRCDVLTTDQSGLYAQRIKMAQPDEFVVLPEVISKEPLGPLVRKGDDEWF
SIVKWTLFAMLNAEEMGITSQNVEEQAKTTKNPDVARLLGADGEYGKDLKLRKDWVVQIV
KQVGNYGEVFERNIGQGSVLKIKRGLNALWNNGGIQYAPPVR