Protein Info for GFF4551 in Xanthobacter sp. DMC5

Annotation: Caffeate CoA-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 7 to 242 (236 residues), 99 bits, E=1.4e-32

Best Hits

Swiss-Prot: 44% identical to YDIF_ECOLI: Acetate CoA-transferase YdiF (ydiF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 67% identity to bgd:bgla_2g26070)

MetaCyc: 49% identical to Pct (Anaerotignum propionicum)
Propionate CoA-transferase. [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (6/7 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• pyruvate fermentation to acetone (4/5 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• (R)-lactate fermentation to propanoate II (acrylate pathway) (2/4 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (3/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (5/9 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• succinate fermentation to butanoate (2/7 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Styrene degradation

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.8

Use Curated BLAST to search for 2.8.3.1 or 2.8.3.8

Protein Sequence (534 amino acids)

>GFF4551 Caffeate CoA-transferase (Xanthobacter sp. DMC5)
MAMRVLSADEAALLVESGDTLLIGGSGGGHAVPDTLIAAVAERFLKEGAPRGITTVHPVG
LGDRKARGIGHLAHDGLLKRVVCGTLVDSPPVEKMALEDRVEAYTLPQGALSQLTREMAS
GRPGLVTHVGLNTFIDPRLGGGRQSPSATEDLVEVVELAGREWLFYKPFVPNIAFLRGTT
ADEDGNISMEQEAIFGEMISMAQATKRAGGIVIVQVKRLARRGTLNPKTVKIPGILVDVV
VVDPGQMQTYVSDYNPAYAGELKIPLSAIAPLPFDPRKVIARRAALELYAGAVCNLGSGI
STGIATVAAEEGCLDAITLTNEQGLIGGAPASGNEVGASRNFHAMIDQPYQFDFYDGGGL
DIAFLSFAEVDAQGSVNVSRFSDRIIGPGGFINISQNAKKVVFSGTFTSGGLDMAWPDGQ
TVIRKEGRDLKFVDRLQQLTYSGAYAAGIGQEALYVTERAVFRRGASGRLELIEIAPGIN
LERDVLAHMDFRPDIAGDLRPMDAALFLPQSIGLEQRLAVSQRSPRGPGARLSA