Protein Info for GFF4540 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Lipoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00510: lipoyl synthase
  • amino acids 21 to 321 (301 residues), 537.8 bits, E=3.6e-166
• PF16881: LIAS_N
  • amino acids 26 to 73 (48 residues), 28.2 bits, 2.2e-10
• PF04055: Radical_SAM
  • amino acids 89 to 252 (164 residues), 81.3 bits, E=1e-26

Best Hits

Swiss-Prot: 100% identical to LIPA_SALSV: Lipoyl synthase (lipA) from Salmonella schwarzengrund (strain CVM19633)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 100% identity to sty:STY0683)

MetaCyc: 97% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

• lipoate biosynthesis and incorporation I (2/2 steps found)
• lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
• lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
• lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)
• lipoate biosynthesis and incorporation II (1/3 steps found)

KEGG Metabolic Maps

• Lipoic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Protein Sequence (321 amino acids)

>GFF4540 Lipoate synthase (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSKPIVMERGVKYRDADKMALIPVKNVVTERDALLRKPEWMKIKLPADSTRIQGIKAAMR
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDAEEPQKLA
QTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIRAKSPEIKIETLVPDFRGRMDRAL
DILNATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
TNAEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPEEFDEMKAEALAMGFTHAA
CGPFVRSSYHADLQAKGMEVK