Protein Info for GFF4539 in Sphingobium sp. HT1-2

Annotation: GTP-binding protein EngA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 TIGR03594: ribosome-associated GTPase EngA" amino acids 3 to 446 (444 residues), 532.6 bits, see alignment E=1.2e-163 TIGR00231: small GTP-binding protein domain" amino acids 4 to 157 (154 residues), 81 bits, see alignment E=1.3e-26 amino acids 183 to 355 (173 residues), 77.5 bits, see alignment E=1.5e-25 PF01926: MMR_HSR1" amino acids 4 to 120 (117 residues), 105.1 bits, see alignment E=1.3e-33 amino acids 186 to 308 (123 residues), 73.5 bits, see alignment E=8.2e-24 PF02421: FeoB_N" amino acids 4 to 124 (121 residues), 43.6 bits, see alignment E=1.3e-14 amino acids 185 to 354 (170 residues), 44.6 bits, see alignment E=6.1e-15 PF00009: GTP_EFTU" amino acids 185 to 357 (173 residues), 44.1 bits, see alignment E=1e-14 PF14714: KH_dom-like" amino acids 366 to 446 (81 residues), 104.9 bits, see alignment E=1.2e-33

Best Hits

Swiss-Prot: 72% identical to DER_NOVAD: GTPase Der (der) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K03977, GTP-binding protein (inferred from 91% identity to sch:Sphch_0055)

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (454 amino acids)

>GFF4539 GTP-binding protein EngA (Sphingobium sp. HT1-2)
MLPTVAIVGRPNVGKSTLFNRLVGKKLALVDDQPGVTRDRREGEAHLLGLDFTIIDTAGY
EDEDPHSLPGRMRMQTQAAVENCDVALFMVDARAGITPLDEEIARWLREGDAPVILVANK
AEGKATEAGVMEAFILGLGEPIPFSAEHGQGLADLFQALLPYLEREDGEEHEKEFYEDEE
SAPLKLAIVGRPNAGKSTLINRLLGENRLLTGPEAGITRDSIAVDWMWTSYEGEERPVRL
IDTAGMRKRAKVQDKLEKLAVSDGLNAVNFAEVVVLLLDATRGLEAQDLRIADKVLEEGR
ALIVALNKWDTVEHGSALYQGIKQALFDGLAQVRGVPIMTVSAATGKGLDDLIKVAFETR
TAWSQRISTGVLNRWFEAALEANPPPAPGGKRIKLRYITQNKTRPPTFVLFGTRLDELPE
SYRRYLVNGIRKELGFGAVPVRLTLRSAKNPYAK