Protein Info for GFF4536 in Xanthobacter sp. DMC5

Annotation: 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 transmembrane" amino acids 756 to 774 (19 residues), see Phobius details PF13806: Rieske_2" amino acids 3 to 99 (97 residues), 35.3 bits, see alignment E=3.3e-12 PF00355: Rieske" amino acids 5 to 88 (84 residues), 62.7 bits, see alignment E=9e-21 amino acids 586 to 668 (83 residues), 59.3 bits, see alignment E=9.7e-20 PF07992: Pyr_redox_2" amino acids 130 to 425 (296 residues), 210.7 bits, see alignment E=1.1e-65 PF00070: Pyr_redox" amino acids 272 to 342 (71 residues), 51.5 bits, see alignment 4.3e-17 PF14759: Reductase_C" amino acids 445 to 516 (72 residues), 50 bits, see alignment 1.3e-16 PF00848: Ring_hydroxyl_A" amino acids 738 to 956 (219 residues), 58.3 bits, see alignment E=3.6e-19

Best Hits

Predicted SEED Role

"Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (958 amino acids)

>GFF4536 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component (Xanthobacter sp. DMC5)
MGFRKVAELDSLRDGGVTGVEVDGVPIALFRMGNEVHATHGICTHALAFLKDGYVEDGTI
ECPLHQGVFDIRTGRALCAPLTRDLATYAAKVEGGAVLVDLESASESPAAAGEADAAQAI
EITDVEPLPLVIAGAGQAAAAAIRAARAAGFSAPIHLVGAEPHLPYERPPLSKEALGDAF
DPASACRLPPADLAALGVTFHPGVRVTAVDAGARVAILSDGTEIPYAALLIATGGTARRL
GVPGDDLPGVHHLRTLEDAQSIAASLASARTVAVVGGGFIGLELASAARARGLKVVLLER
EGELMSRLLPPALGAAFRRLAQANGVDVRRDVAVEAITARDEALALATTAGEVVADTVLV
GVGLVPETGLAALAGCEVAGGVVVDSAGRTSVEGIWAAGDCALHHAPLNGRRGRLESWQN
AEEQGAAAGRSIAGDDTIRAEKAPWFWTDQFGLNIQMLGVPATTDEVALSGTDGAAGAVY
RTLADGRVTAVTAFSDAAAIRAARAELASDTPFDLAAHGARLLAPGEFEPTPTDPEEDDM
SASPDVQFAKKYVWPQEGLSIIPDWVYTDETVYQREVERIFHGRTWNFVALECEIENPGD
FIRSNVGPTPVVVARAEDGTIHVFENRCAHRAAEFCRDLSGNAKEFVCPYHQWSYDLKGN
LVGVPFRRGVDGKGGMPKDFKPADHGLKQLNVTTHRGVVFASYADGMESFPDYLGPEVLR
EFEATFDGRKPKLLGHYRHSLPGNWKLYHENLKDPYHATLLHTFLVTFGLLVAGNKSLML
ADPSGRHGVMASAKSDTTHIDADKKKEMRAYKEGMTLEEPRFMDFIDEFDSPWSVTMATI
WPNLIVQREMNTLGIRHIVPTGPNEFIMKWTMFGFEGDDEEMTRHRLRQGNLMGPAGFLG
LEDNEAIKFVQDGMLGVPGGEHLVKLDPGTEAGTADTLISEAAIRAMYRHWRDAMGIA