Protein Info for GFF4533 in Variovorax sp. SCN45

Annotation: Multidrug resistance transporter, Bcr/CflA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 transmembrane" amino acids 17 to 39 (23 residues), see Phobius details amino acids 47 to 70 (24 residues), see Phobius details amino acids 82 to 100 (19 residues), see Phobius details amino acids 106 to 128 (23 residues), see Phobius details amino acids 141 to 164 (24 residues), see Phobius details amino acids 171 to 189 (19 residues), see Phobius details amino acids 217 to 242 (26 residues), see Phobius details amino acids 255 to 276 (22 residues), see Phobius details amino acids 284 to 304 (21 residues), see Phobius details amino acids 310 to 332 (23 residues), see Phobius details amino acids 348 to 367 (20 residues), see Phobius details amino acids 373 to 395 (23 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 15 to 390 (376 residues), 255.5 bits, see alignment E=5.8e-80 PF07690: MFS_1" amino acids 20 to 360 (341 residues), 163.9 bits, see alignment E=7.4e-52 PF00083: Sugar_tr" amino acids 52 to 193 (142 residues), 47 bits, see alignment E=2.8e-16 PF06609: TRI12" amino acids 52 to 176 (125 residues), 25 bits, see alignment E=9.6e-10

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 87% identity to vpe:Varpa_0913)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (409 amino acids)

>GFF4533 Multidrug resistance transporter, Bcr/CflA family (Variovorax sp. SCN45)
MSGAATPEPQRRPHAPLWLLALVTFSGTLAMHIFVPALPIAAANLGAGIGAMQMTVSLYI
FGLAVGQLVYGPLADRFGRRPVLLCGLAIYCVAGLAAALAPEVHALITARLFQAIGGCAG
LVLGRSIVRDTAEPQEATRRLALMNLMVTIGPSLAPLIGGALATALGWRSIFYALFGLGV
VGFLFTWRMLPETGAPGAAVNARDLARNYGKLLRSPAFLGFSVGGGCATTSMYAFIASAP
FIFVDQLHRPAHEAGIYLAILVSGVWLGSFLTSHLISRVRVDRLMIRSNALSVAAAFVLL
GAALGDHLSVPLIVACMFLFTVGAGMASPAALTQAISVNPQVIGSASGLYGFTQMAVGAL
CTALAGMGHRNPALASAIVLAGAGIVAQLSFAVALRTQRRTAAAATLSA